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Liver Arginase Recombinant Rabbit Monoclonal Antibody [JB21-49]

Usd: 385

Specification

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Catalog# ET7107-90

Liver Arginase Recombinant Rabbit Monoclonal Antibody [JB21-49]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Liver Arginase Recombinant Rabbit Monoclonal Antibody [JB21-49]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Liver Arginase aa 273-322 / 322.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Human liver tissue lysates, human liver tissue.

Conjugation

unconjugated

Clone Number

JB21-49

RRID

AB_3070795

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:15,000

  • IP

  • 1:10-1:50

Target

Function

Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence..

Background References

1. Munder M et al. Arginase I is constitutively expressed in human granulocytes and participates in fungicidal activity. Blood 105:2549-2556 (2005).

2. Munder M et al. Suppression of T-cell functions by human granulocyte arginase. Blood 108:1627-1634 (2006).

Sequence Similarity

Belongs to the arginase family.

Tissue Specificity

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P05089 Human

Synonyms

A I antibody

Al antibody

ARG 1 antibody

arg1 antibody

ARGI1_HUMAN antibody

Arginase 1 antibody

Arginase liver antibody

Arginase type I antibody

Arginase, liver antibody

Arginase-1 antibody

Expand

Images

  • Western blot analysis of Liver Arginase on human liver tissue lysates with Rabbit anti-Liver Arginase antibody (<a href="/products/ET7107-90" style="font-weight: bold;text-decoration: underline;">ET7107-90</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-90" style="font-weight: bold;text-decoration: underline;">ET7107-90</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Liver Arginase on human liver tissue lysates with Rabbit anti-Liver Arginase antibody (ET7107-90) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 37 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody (<a href="/products/ET7107-90" style="font-weight: bold;text-decoration: underline;">ET7107-90</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-90" style="font-weight: bold;text-decoration: underline;">ET7107-90</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Arginase antibody (ET7107-90) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-90) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Liver Arginase antibody (<a href="/products/ET7107-90" style="font-weight: bold;text-decoration: underline;">ET7107-90</a>) at 1/15,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-90" style="font-weight: bold;text-decoration: underline;">ET7107-90</a>) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-Liver Arginase antibody (ET7107-90) at 1/15,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-90) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"