PARP2 Recombinant Rabbit Monoclonal Antibody [JG34-56]
Catalog# ET7108-05
PARP2 Recombinant Rabbit Monoclonal Antibody [JG34-56]
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WB
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Human
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unconjugated
Overview
Product Name
PARP2 Recombinant Rabbit Monoclonal Antibody [JG34-56]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human PARP2 aa 484-583 / 583.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 66 kDa
Positive Control
HCT 116 cell lysate, HeLa cell lysate.
Conjugation
unconjugated
Clone Number
JG34-56
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000-1:2,000
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity.
Background References
1. Ame J.-C. et al. PARP-2, a novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase. J. Biol. Chem. 274:17860-17868 (1999).
2. Bonfiglio J.J. et al. Serine ADP-ribosylation depends on HPF1. Mol. Cell 0:0-0 (2017).
Tissue Specificity
Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
Post-translational Modification
Poly-ADP-ribosylated by PARP1.; Acetylation reduces DNA binding and enzymatic activity.
Subcellular Location
Chromosome, Nucleus.
UNIPROT
Synonyms
ADP ribosyltransferase like 2 antibody
ADP-ribosyltransferase diphtheria toxin-like 2 antibody
ADPRT 2 antibody
ADPRT-2 antibody
ADPRT2 antibody
ADPRTL 2 antibody
ADPRTL 3 antibody
ADPRTL2 antibody
ADPRTL3 antibody
ARTD2 antibody
ExpandImages
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Western blot analysis of PARP2 on different lysates with Rabbit anti-PARP2 antibody (ET7108-05) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-05) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of PARP2 on different lysates with Rabbit anti-PARP2 antibody (ET7108-05) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si PARP2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 55 kDa
Exposure time: 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-05) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"