Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]
Catalog# ET7108-07
Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]
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WB
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FC
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Human
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unconjugated
Overview
Product Name
Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Nuf2 aa 1-108 / 464.
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 54 kDa
Positive Control
HEK-293 cell lysates.
Conjugation
unconjugated
Clone Number
JG34-64
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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FC
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1:50-1:100
Target
Function
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
Background References
1. Schmidt J.C. et al. The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments. Dev. Cell 23:968-980 (2012).
2. Liu D. et al. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J. Biol. Chem. 282:21415-21424(2007).
Sequence Similarity
Belongs to the NUF2 family.
Post-translational Modification
Can be phosphorylated by AURKA and AURKB.
Subcellular Location
Centromere, Chromosome, Kinetochore, Nucleus.
UNIPROT
Synonyms
Cancer/testis antigen 106 antibody
CDCA 1 antibody
CDCA1 antibody
Cell division cycle associated 1 antibody
Cell division cycle associated protein 1 antibody
Cell division cycle-associated protein 1 antibody
CT106 antibody
hNuf 2 antibody
hNuf2 antibody
hNuf2R antibody
ExpandImages
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Western blot analysis of Nuf2 on HEK-293 cell lysate with Rabbit anti-Nuf2 antibody (ET7108-07) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 60 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7108-07, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 54 kDa
Observed band size: 54 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"