Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64]

Function

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

Background References

1. Schmidt J.C. et al. The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments. Dev. Cell 23:968-980 (2012).

2. Liu D. et al. Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment. J. Biol. Chem. 282:21415-21424(2007).

Sequence Similarity

Belongs to the NUF2 family.

Post-translational Modification

Can be phosphorylated by AURKA and AURKB.

Subcellular Location

Centromere, Chromosome, Kinetochore, Nucleus.

UNIPROT

SwissProt: Q9BZD4 Human

Synonyms