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Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]

Usd: 385

Specification

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Catalog# ET7108-13

Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]

Application

  • WB

  • IHC-P

  • FC

  • IP

  • IF-Cell

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Pumilio 1 Recombinant Rabbit Monoclonal Antibody [JG37-75]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Pumilio 1 aa 79-223 .

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC, IP, IF-Cell, IF-Tissue

Target Molecular Weight

Predicted band size: 126 kDa

Positive Control

SiHa cell lysate, NCCIT cell lysate, 293T, SiHa, human colon tissue, human appendix tissue, mouse kidney tissue, mouse small intestine tissue.

Conjugation

unconjugated

Clone Number

JG37-75

RRID

AB_3070813

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

Target

Function

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs.

Background References

1. Lee S et al. Noncoding RNA NORAD regulates genomic stability by sequestering PUMILIO proteins. Cell 164:69-80 (2016).

2. Narita R et al. A novel function of human Pumilio proteins in cytoplasmic sensing of viral infection. PLoS Pathog 10:E1004417-E1004417 (2014).

Tissue Specificity

Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.

Post-translational Modification

Phosphorylation at Ser-714 promotes RNA-binding activity. Following growth factor stimulation phosphorylated at Ser-714, promoting binding to the 3'-UTR of CDKN1B/p27 mRNA.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: Q14671 Human

SwissProt: Q80U78 Mouse

Synonyms

HsPUM antibody

KIAA0099 antibody

PUM 1 antibody

PUM L1 antibody

Pum1 antibody

PUM1_HUMAN antibody

PUMH 1 antibody

PUMH antibody

PUMH1 antibody

pumilio homolog 1 (Drosophila) antibody

Expand

Images

  • Western blot analysis of Pumilio 1 on different lysates with Rabbit anti-Pumilio 1 antibody (<a href="/products/ET7108-13" style="font-weight: bold;text-decoration: underline;">ET7108-13</a>) at 1/1,000 dilution.<br /><br />Lane 1: SiHa cell lysate<br />Lane 2: NCCIT cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 25 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7108-13" style="font-weight: bold;text-decoration: underline;">ET7108-13</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 126 kDa<br />Observed band size: 126 kDa

    Western blot analysis of Pumilio 1 on different lysates with Rabbit anti-Pumilio 1 antibody (ET7108-13) at 1/1,000 dilution.

    Lane 1: SiHa cell lysate
    Lane 2: NCCIT cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 25 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7108-13, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 126 kDa
    Observed band size: 126 kDa

  • ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"