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Syntrophin alpha 1 Recombinant Rabbit Monoclonal Antibody [JG35-62]

Usd: 385

Specification

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Catalog# ET7108-15

Syntrophin alpha 1 Recombinant Rabbit Monoclonal Antibody [JG35-62]

Application

  • WB

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Syntrophin alpha 1 Recombinant Rabbit Monoclonal Antibody [JG35-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Syntrophin alpha 1 aa 1-49 / 505.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC, IF-Tissue

Molecular Weight

Predicted band size: 54 kDa

Positive Control

SiHa cell lysate, NIH/3T3 cell lysate, human skeletal muscle tissue, rat skeletal muscle tissue, mouse colon tissue, human colon tissue, LOVO.

Conjugation

unconjugated

Clone Number

JG35-62

RRID

AB_3070815

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

Target

Function

The syntrophins are PDZ-domain-containing proteins that facilitate the recruitment of signaling proteins such as NOS1 to the dystrophin-associated protein complex. The syntrophin family are structurally related proteins that contain multiple protein interaction motifs. Syntrophins associate directly with dystrophin, the product of the Duchenne muscular dystrophy locus, and its homologs. α-Syntrophin has an important role in synapse formation and in the organization of utrophin, acetylcholine receptor and acetylcholinester-ase at the neuromuscular synapse. Specifically, NOS1 binds to α-Syntrophin at muscle sarcolemma. β2-Syntrophin is a modular adapter. In muscle cells, β2-Syntrophin interacts with members of the dystrophin family, which in-cludes utrophin.

Background References

1. Wu G et al. alpha-1-syntrophin mutation and the long-QT syndrome: a disease of sodium channel disruption. Circ Arrhythm Electrophysiol 1:193-201 (2008).

2. Ueda K et al. Syntrophin mutation associated with long QT syndrome through activation of the nNOS-SCN5A macromolecular complex. Proc Natl Acad Sci USA 105:9355-9360 (2008).

Sequence Similarity

Belongs to the syntrophin family.

Tissue Specificity

High expression in skeletal muscle and heart. Low expression in brain, pancreas, liver, kidney and lung. Not detected in placenta.

Post-translational Modification

Phosphorylated by CaM-kinase II. Phosphorylation may inhibit the interaction with DMD (By similarity).

Subcellular Location

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane.

UNIPROT

SwissProt: Q13424 Human

SwissProt: Q61234 Mouse

Entrez Gene: 362242 Rat

Synonyms

59 kDa dystrophin-associated protein A1 acidic component 1 antibody

Acidic alpha 1 syntrophin antibody

Alpha 1 syntrophin antibody

Alpha-1-syntrophin antibody

dJ1187J4.5 antibody

Dystrophin associated protein A1 59kDa acidic component antibody

LQT12 antibody

OTTHUMP00000030650 antibody

Pro-TGF-alpha cytoplasmic domain-interacting protein 1 antibody

SNT1 antibody

Expand

Images

  • Western blot analysis of Syntrophin alpha 1 on different lysates with Rabbit anti-Syntrophin alpha 1 antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>) at 1/1,000 dilution.<br /><br />Lane 1: SiHa cell lysate<br />Lane 2: NIH/3T3 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 1 minute 21 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Syntrophin alpha 1 on different lysates with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/1,000 dilution.

    Lane 1: SiHa cell lysate
    Lane 2: NIH/3T3 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 1 minute 21 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-15) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence analysis of paraffin-embedded human colon tissue labeling Syntrophin alpha 1 with Rabbit anti-Syntrophin alpha 1 antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded human colon tissue labeling Syntrophin alpha 1 with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7108-15, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Syntrophin alpha 1 antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Syntrophin alpha 1 antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-15" style="font-weight: bold;text-decoration: underline;">ET7108-15</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Syntrophin alpha 1 antibody (ET7108-15) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation