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KAT8 Recombinant Rabbit Monoclonal Antibody [JG36-05]

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Specification

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Catalog# ET7108-23

KAT8 Recombinant Rabbit Monoclonal Antibody [JG36-05]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Rat

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

KAT8 Recombinant Rabbit Monoclonal Antibody [JG36-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human KAT8 aa 310-458 / 458.

Species Reactivity

Human, Rat, Mouse

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Molecular Weight

Predicted band size: 52 kDa

Positive Control

HEK-293 cell lysate, HepG2 cell lysate, PC-3M cell lysate, MCF-7, SH-SY-5Y, SiHa, human cervix tissue, human cervix cancer tissue, rat cervix tissue, K562.

Conjugation

unconjugated

Clone Number

JG36-05

RRID

AB_3070823

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:1,000

Target

Function

K(lysine) acetyltransferase 8 (KAT8) is an enzyme that in humans is encoded by the KAT8 gene.The MYST family of histone acetyltransferases, which includes KAT8, was named for the founding members MOZ (MYST3; MIM 601408), yeast YBF2 and SAS2, and TIP60 (HTATIP; MIM 601409). All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein-protein interactions and targeting transcriptional regulators to chromatin.

Background References

1. Neal K C et al. A new human member of the MYST family of histone acetyl transferases with high sequence similarity to Drosophila MOF. Biochim Biophys Acta 1490:170-174 (2000).

2. Cai Y et al. Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. J Biol Chem 285:4268-4272 (2010).

Sequence Similarity

Belongs to the MYST (SAS/MOZ) family.

Post-translational Modification

Autoacetylation at Lys-274 is required for binding histone H4 with high affinity and for proper function.

Subcellular Location

Chromosome. Nucleus.

UNIPROT

SwissProt: Q9H7Z6 Human

SwissProt: Q9D1P2 Mouse

SwissProt: Q5XI06 Rat

Synonyms

Histone acetyltransferase KAT8 antibody

Histone acetyltransferase MYST1 antibody

hMOF antibody

K(lysine) acetyltransferase 8 antibody

KAT 8 antibody

Lysine acetyltransferase 8 antibody

MOF antibody

MOZ antibody

MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1 antibody

MYST 1 antibody

Expand

Images

  • Western blot analysis of KAT8 on different lysates with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: PC-3M cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 52 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of KAT8 on different lysates with Rabbit anti-KAT8 antibody (ET7108-23) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: PC-3M cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 52 kDa
    Observed band size: 55 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-23) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of MCF-7 cells labeling KAT8 with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of MCF-7 cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of SH-SY-5Y cells labeling KAT8 with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SH-SY-5Y cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of SiHa cells labeling KAT8 with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SiHa cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cervix tissue with Rabbit anti-KAT8 antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-23" style="font-weight: bold;text-decoration: underline;">ET7108-23</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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Citation