WSTF Recombinant Rabbit Monoclonal Antibody [JG36-32]
Catalog# ET7108-32
WSTF Recombinant Rabbit Monoclonal Antibody [JG36-32]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
WSTF Recombinant Rabbit Monoclonal Antibody [JG36-32]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human WSTF aa 2-96 / 1,483.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 171 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, L-929 cell lysate, PC-12 cell lysate, HeLa, NIH/3T3.
Conjugation
unconjugated
Clone Number
JG36-32
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:100-1:250
Target
Function
Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication.
Background References
1. Cook P.J. et al. Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions. Nature 458:591-596(2009).
2. Xiao A. et al. WSTF regulates the H2A.X DNA damage response via a novel tyrosine kinase activity. Nature 457:57-62(2009).
Sequence Similarity
Belongs to the WAL family. BAZ1B subfamily.
Tissue Specificity
Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.
Subcellular Location
Nucleus.
Synonyms
baz1b antibody
BAZ1B_HUMAN antibody
Bromodomain adjacent to zinc finger domain protein 1B antibody
hWALP 2 antibody
hWALP-2 antibody
hWALP2 antibody
transcription factor WSTF antibody
Tyrosine-protein kinase BAZ1B antibody
WALP-2 antibody
WALP2 antibody
ExpandImages
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Western blot analysis of WSTF on different lysates with Rabbit anti-WSTF antibody (ET7108-32) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: L-929 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 171 kDa
Observed band size: 171 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-32) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling WSTF with Rabbit anti-WSTF antibody (ET7108-32) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WSTF antibody (ET7108-32) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling WSTF with Rabbit anti-WSTF antibody (ET7108-32) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WSTF antibody (ET7108-32) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"