SF3B3 Recombinant Rabbit Monoclonal Antibody [JG63-53]
Catalog# ET7108-36
SF3B3 Recombinant Rabbit Monoclonal Antibody [JG63-53]
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SF3B3 Recombinant Rabbit Monoclonal Antibody [JG63-53]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SF3B3 aa 1061-1217 / 1217.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
136 kDa
Positive Control
Jurkat cell lysate, Hela cell lysate, human colon cancer tissue, human kidney tissue, mouse testis tissue, human tonsil tissue, rat brain tissue, LOVO.
Conjugation
unconjugated
Clone Number
JG63-53
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.
Background References
1. Das B K et al. Characterization of a protein complex containing spliceosomal proteins saps 49,130,145 and 155. Mol Cell Biol 19:6796-6802 (1999).
2. Das R et al. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell 5:779-787 (2000).
Sequence Similarity
Belongs to the RSE1 family.
Subcellular Location
Nucleus. Spliceosome.
Synonyms
KIAA0017 antibody
Pre mRNA splicing factor SF3b 130 kDa subunit antibody
Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
RSE1 antibody
SAP 130 antibody
SAP130 antibody
SF3b130 antibody
SF3B3 antibody
SF3B3_HUMAN antibody
Spliceosome associated protein 130 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SF3B3 antibody (ET7108-36) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"