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PKC gamma Recombinant Rabbit Monoclonal Antibody [JG69-32]

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Specification

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Catalog# ET7108-42

PKC gamma Recombinant Rabbit Monoclonal Antibody [JG69-32]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PKC gamma Recombinant Rabbit Monoclonal Antibody [JG69-32]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human PKC gamma aa 564-697 / 697.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 78 kDa

Positive Control

Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, A549 cell lysates, mouse cerebellum tissue, rat cerebellum tissue.

Conjugation

unconjugated

Clone Number

JG69-32

RRID

AB_3070840

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:1,000-1:5,000

Target

Function

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η, θ, λ/ι, µ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Background References

1. Yoshida K et al. Protein kinase C delta regulates Ser46 phosphorylation of p53 tumor suppressor in the apoptotic response to DNA damage. J Biol Chem 281:5734-5740 (2006).

2. Chen D-H et al. Missense mutations in the regulatory domain of PKC gamma: a new mechanism for dominant nonepisodic cerebellar ataxia. Am J Hum Genet 72:839-849 (2003).

Sequence Similarity

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Tissue Specificity

Expressed in Purkinje cells of the cerebellar cortex.

Post-translational Modification

Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4.; Ubiquitinated.

Subcellular Location

Cell membrane. Cytoplasm.

UNIPROT

SwissProt: P05129 Human

SwissProt: P63318 Mouse

SwissProt: P63319 Rat

Synonyms

KPCG_HUMAN antibody

MGC57564 antibody

OTTHUMP00000067291 antibody

PKC-gamma antibody

PKCC antibody

PKCG antibody

PRKCG antibody

Protein kinase C gamma antibody

Protein kinase C gamma polypeptide antibody

Protein kinase C gamma type antibody

Expand

Images

  • Western blot analysis of PKC gamma on different lysates with Rabbit anti-PKC gamma antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse cerebellum tissue lysate<br />Lane 3: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PKC gamma on different lysates with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse cerebellum tissue lysate
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 75 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PKC gamma on A549 cell lysate lysates with Rabbit anti-PKC gamma antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 78 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PKC gamma on A549 cell lysate lysates with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 78 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-42) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PKC gamma antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-42) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PKC gamma antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-42" style="font-weight: bold;text-decoration: underline;">ET7108-42</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-42) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation