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Tetranectin Recombinant Rabbit Monoclonal Antibody [JG37-16]

Usd: 385

Specification

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Catalog# ET7108-45

Tetranectin Recombinant Rabbit Monoclonal Antibody [JG37-16]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Tetranectin Recombinant Rabbit Monoclonal Antibody [JG37-16]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Tetranectin aa 110-202 / 202.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 23 kDa

Positive Control

Rat skin tissue lysate, Human skin tissue lysate, human liver tissue, human kidney tissue, rat liver tissue, HepG2, MCF-7.

Conjugation

unconjugated

Clone Number

JG37-16

RRID

AB_3070843

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:200-1:1,000

  • IP

  • 1:10-1:50

Target

Function

Tetranectin is a homotrimeric glycoprotein present in plasma and various tissue locations that binds to calcium, heparin, and plasminogen kringle 4. Tetranectin may play a prominent role in tissue remodeling as well as in the regulation of proteolytic processes via its binding and indirect activation of plasminogen. Tetranectin is found in the extracellular matrix (ECM) of certain carcinomas, but is not present in the ECM of normal tissues. Extracellular proteolysis is an important factor in the ability of malignant cells to penetrate normal tissues and metastasize. Decreased plasma tetranectin or increased tetranectin in stroma of cancers correlates with cancer progression and a grim prognosis. Tetranectin may also influence cancer growth by altering activities of plasminogen or the plasminogen fragment, angiostatin which inhibits tumor cell proliferation.

Background References

1. Fuhlendorff J et al. Primary structure of tetranectin, a plasminogen kringle 4 binding plasma protein: homology with asialoglycoprotein receptors and cartilage proteoglycan core protein. Biochemistry 26:6757-6764 (1987).

2. Jaquinod M et al. Mass spectrometric characterisation of post-translational modification and genetic variation in human tetranectin. Biol Chem 380:1307-1314 (1999).

Tissue Specificity

Found in plasma.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P05452 Human

SwissProt: P43025 Mouse

Entrez Gene: 316099 Rat

Synonyms

C type lectin domain family 3 member B antibody

C-type lectin domain family 3 member B antibody

Clec3b antibody

Plasminogen kringle 4 binding protein antibody

Plasminogen kringle 4-binding protein antibody

TETN_HUMAN antibody

Tetranectin (plasminogen binding protein) antibody

Tetranectin antibody

TN antibody

TNA antibody

Images

  • Western blot analysis of Tetranectin on different lysates with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/500 dilution.<br /><br />Lane 1: Rat skin tissue lysate<br />Lane 2: Human skin tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 23 kDa<br />Observed band size: 23 kDa<br /><br />Exposure time: 2 minutes;<br />15% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Tetranectin on different lysates with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/500 dilution.

    Lane 1: Rat skin tissue lysate
    Lane 2: Human skin tissue lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

    Exposure time: 2 minutes;
    15% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-45) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of HepG2 cells labeling Tetranectin with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of HepG2 cells labeling Tetranectin with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of MCF-7 cells labeling Tetranectin with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tetranectin antibody (<a href="/products/ET7108-45" style="font-weight: bold;text-decoration: underline;">ET7108-45</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of MCF-7 cells labeling Tetranectin with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tetranectin antibody (ET7108-45) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

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