Fibrinogen gamma chain Recombinant Rabbit Monoclonal Antibody [JG37-18]
Catalog# ET7108-46
Fibrinogen gamma chain Recombinant Rabbit Monoclonal Antibody [JG37-18]
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Fibrinogen gamma chain Recombinant Rabbit Monoclonal Antibody [JG37-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Fibrinogen gamma chain aa 361-453 / 453.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
52 kDa
Positive Control
Human plasma lysates, human colon tissue, HepG2.
Conjugation
unconjugated
Clone Number
JG37-18
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Together with fibrinogen alpha (FGA) and fibrinogen beta (FGB), polymerizes to form an insoluble fibrin matrix. Has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.
Background References
1. Everse S.J. et al. Conformational changes in fragments D and double-D from human fibrin(ogen) upon binding the peptide ligand Gly-His-Arg-Pro-amide. Biochemistry 38:2941-2946(1999).
2. Kollman J.M. et al. Crystal structure of human fibrinogen. Biochemistry 48:3877-3886(2009).
Tissue Specificity
Detected in blood plasma (at protein level).
Post-translational Modification
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.; Sulfation of C-terminal tyrosines increases affinity for thrombin.
Subcellular Location
Secreted.
UNIPROT
Synonyms
FGG antibody
FIBG_HUMAN antibody
Fibrinogen gamma chain antibody
Fibrinogen gamma polypeptide antibody
fibrinogen gamma-b chain antibody
Images
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Western blot analysis of Fibrinogen gamma chain on human plasma lysates lysates with Rabbit anti-Fibrinogen gamma chain antibody (ET7108-46) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-46) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Fibrinogen gamma chain antibody (ET7108-46) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-46) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Fibrinogen gamma chain was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-46, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"