P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]
Catalog# ET7108-52
P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]
-
WB
-
IP
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
P2Y6 Recombinant Rabbit Monoclonal Antibody [JG37-77]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human P2Y6 aa 279-328 / 328.
Species Reactivity
Human
Validated Applications
WB, IP, IHC-P
Target Molecular Weight
Predicted band size: 36 kDa
Positive Control
A549, human liver tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
JG37-77
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IP
-
1:10-1:50
-
IHC-P
-
1:200-1:1,000
Target
Function
The product of this gene, P2Y6, belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. Four transcript variants encoding the same isoform have been identified for this gene.
Background References
1. Communi D et al. Cloning, functional expression and tissue distribution of the human P2Y6 receptor. Biochemical and Biophysical Research Communications 222(2): 303-8 (1996).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
G coupled nucleotide receptor antibody
G protein coupled 6 antibody
MGC15335 antibody
P2 purinoceptor antibody
P2ry6 antibody
P2RY6_HUMAN antibody
P2Y purinoceptor 6 antibody
P2Y6 antibody
P2Y6 receptor antibody
PP2891 antibody
ExpandImages
-
Western blot analysis of P2Y6 on A549 (Human lung adenocarcinoma cell) cell lysate with Rabbit anti-P2Y6 antibody (ET7108-52) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 62 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7108-52, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 36.4 kDa
Observed band size: 40 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Liver
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET7108-52, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Kidney
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: ET7108-52, 1/200, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"