PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]
Catalog# ET7108-60
PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]
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WB
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IP
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FC
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
PICK1 Recombinant Rabbit Monoclonal Antibody [JG30-80]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PICK1 aa 1-50 / 415.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IP, FC
Molecular Weight
47 kDa
Positive Control
SK-Br-3 cell lysate, 293 cell lysate, 293T.
Conjugation
unconjugated
Clone Number
JG30-80
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
Target
Function
Protein interacting with C kinase 1 (PICK1) is a PDZ-domain containing protein that is located in the perinuclear region and is phosphorylated in response to PKCα activation. PKCα, which is essential for the regulation of proliferation and differentiation in numerous cell types, contains within its catalytic region a PDZ-binding domain that is absent from other PKC isoforms. Mutation of the PICK1 PDZ domain inhibits the binding of PICK1 to PKCα. PICK1 also interacts with the carboxy terminus of a-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor, a neurotransmitter receptor located at excitatory synapses, suggesting that PICK1 plays a role in the modulation of synaptic transmission by targeting and anchoring AMPA to specific synapses.
Background References
1. Shao X et al. Threonine 82 at the PDZ domain of PICK1 is critical for AMPA receptor interaction and localization. Neurochem Int 56:962-970 (2010).
2. Takeya R et al. Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors. Biochem Biophys Res Commun 267:149-155 (2000).
Tissue Specificity
Ubiquitous.
Post-translational Modification
Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors.; Palmitoylation on Cys-413 is essential for long-term synaptic depression (LTD).
Subcellular Location
cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome.
UNIPROT
Synonyms
dJ1039K5 antibody
MGC15204 antibody
OTTHUMP00000028509 antibody
PICK 1 antibody
Pick1 antibody
PICK1_HUMAN antibody
PRKCA binding protein antibody
PRKCA-binding protein antibody
PRKCABP antibody
Protein interacting with C kinase 1 antibody
ExpandImages
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Western blot analysis of PICK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: 293 cell lysate -
Flow cytometric analysis of PICK1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-60, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"