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Tropomyosin 1 Recombinant Rabbit Monoclonal Antibody [JG38-66]

Usd: 385

Specification

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Catalog# ET7108-74

Tropomyosin 1 Recombinant Rabbit Monoclonal Antibody [JG38-66]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Tropomyosin 1 Recombinant Rabbit Monoclonal Antibody [JG38-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Tropomyosin 1 aa 168-284 / 284.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

33 kDa

Positive Control

Rat heart tissue lysate, mouse heart tissue lysate, rat smooth muscle tissue, human heart tissue, mouse skeletal muscle tissue, Hela.

Conjugation

unconjugated

Clone Number

JG38-66

RRID

AB_3070869

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin α is encoded by the TPM1 gene, which maps to human chromosome 15q22.1 and undergoes alternative splicing to generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Tropomyosin β is encoded by the TPM2 gene, which maps to human chromosome 9p13.3 and undergoes alternative splicing to generate three isoforms, including skeletal muscle (isoform 1), non-muscle/fibroblast TM36/epithelial TMe1 (isoform 2) and non-muscle (isoform 3). Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction.

Background References

1. Gimona M et al. Specificity of dimer formation in tropomyosins: influence of alternatively spliced exons on homodimer and heterodimer assembly. Proc Natl Acad Sci USA 92:9776-9780 (1995).

2. Kalyva A et al. In vitro formation and characterization of the skeletal muscle alpha.beta tropomyosin heterodimers. Biochemistry 51:6388-6399 (2012).

Sequence Similarity

Belongs to the tropomyosin family.

Tissue Specificity

Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese patients. Isoform 1 is expressed in adult and fetal skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in adult and fetal cardiac tissues, but not in skeletal muscle.

Post-translational Modification

Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells.

Subcellular Location

Cytoskeleton.

UNIPROT

SwissProt: P09493 Human

SwissProt: P58771 Mouse

SwissProt: P04692 Rat

Synonyms

AA986836 antibody

AI854628 antibody

Alpha tropomyosin antibody

alpha-TM antibody

Alpha-tropomyosin antibody

C15orf13 antibody

cardiomyopathy, hypertrophic 3 antibody

CMD1Y antibody

CMH3 antibody

HTM alpha antibody

Expand

Images

  • Western blot analysis of Tropomyosin 1 on different lysates with Rabbit anti-Tropomyosin 1 antibody (<a href="/products/ET7108-74" style="font-weight: bold;text-decoration: underline;">ET7108-74</a>) at 1/1,000 dilution.<br /><br />Lane 1: Rat heart tissue lysate<br />Lane 2: Mouse heart tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 46 seconds;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-74" style="font-weight: bold;text-decoration: underline;">ET7108-74</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Tropomyosin 1 on different lysates with Rabbit anti-Tropomyosin 1 antibody (ET7108-74) at 1/1,000 dilution.

    Lane 1: Rat heart tissue lysate
    Lane 2: Mouse heart tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 35 kDa

    Exposure time: 46 seconds;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-74) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-74" style="font-weight: bold;text-decoration: underline;">ET7108-74</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-74" style="font-weight: bold;text-decoration: underline;">ET7108-74</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-74" style="font-weight: bold;text-decoration: underline;">ET7108-74</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Tropomyosin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Tropomyosin 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7108-74" style="font-weight: bold;text-decoration: underline;">ET7108-74</a>, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).

    Flow cytometric analysis of Tropomyosin 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7108-74, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).

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Citation