Ube2N Recombinant Rabbit Monoclonal Antibody [JG38-68]

Function

Ube2N, a member of the E2 Ubiquitin-conjugating enzyme family, completes the second step of ubiquitination reactions, essentially targeting proteins for proteasomic degradation. When proteins are modified with ubiquitin in an important cellular mechanism targeting abnormal or short lived proteins, ubiquitin, a short protein of 76 amino acids, attaches to a lysine residue resting on the target protein. Multiple cycles of ubiquitination create a polyubiquitin chain that the proteasome recognizes and subsequently triggers the ATP-dependent unfolding of the target protein. This allows protoeolytic degradation of the target protein. These degradation products are highly expressed in heart and skeletal muscles. Ube2N moderates the transcriptional activation of target genes, affects the progress of cell differentiation and aging, and also influences the DNA repair pathway, further adding to the survival of cells after DNA damage.

Background References

1. Hofmann R M et al. Noncanonical MMS2-encoded ubiquitin-conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair. Cell 96:645-653 (1999).

2. Bothos J et al. The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains. Oncogene 22:7101-7107 (2003).

Sequence Similarity

Belongs to the ubiquitin-conjugating enzyme family.

Post-translational Modification

Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.

Subcellular Location

Nucleus. Cytoplasm.

UNIPROT

SwissProt: P61088 Human

SwissProt: P61089 Mouse

SwissProt: Q9EQX9 Rat

Synonyms