Skip to content

p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET7108-81

p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

p54nrb/NONO Recombinant Rabbit Monoclonal Antibody [JG87-38]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human p54nrb aa 365-471 / 471.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 54 kDa

Positive Control

A549 cell lysate, 293cell lysate, SH-SY-5Y cell lysate, SiHa, human liver tissue, mouse epididymis tissue, mouse testis tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JG87-38

RRID

AB_3070875

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:50-1:200

Target

Function

Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing.

Background References

1. Straub T et al. PSF/p54(nrb) stimulates \'jumping\' of DNA topoisomerase I between separate DNA helices. Biochemistry 39:7552-7558 (2000).

2. Zhang Z et al. The fate of dsRNA in the nucleus: a p54(nrb)-containing complex mediates the nuclear retention of promiscuously A-to-I edited RNAs. Cell 106:465-475 (2001).

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.

Post-translational Modification

The N-terminus is blocked.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q15233 Human

SwissProt: Q99K48 Mouse

SwissProt: Q5FVM4 Rat

Synonyms

52 kDa subunit antibody

54 kDa nuclear RNA and DNA binding protein antibody

54 kDa nuclear RNA- and DNA-binding protein antibody

55 kDa nuclear protein antibody

DNA binding p52/p100 complex 52 kDa subunit antibody

DNA-binding p52/p100 complex antibody

NMT 55 antibody

NMT55 antibody

Non Pou domain containing octamer (ATGCAAAT) binding protein antibody

Non POU domain containing octamer binding antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of p54nrb/NONO on different lysates with Rabbit anti-p54nrb/NONO antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si p54nrb/NONO cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of p54nrb/NONO on different lysates with Rabbit anti-p54nrb/NONO antibody (ET7108-81) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si p54nrb/NONO cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 60 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution.

    Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution.

  • ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-p54nrb/NONO antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-p54nrb/NONO antibody (ET7108-81) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-p54nrb/NONO antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-p54nrb/NONO antibody (ET7108-81) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p54nrb/NONO antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p54nrb/NONO antibody (ET7108-81) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-p54nrb/NONO antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-81" style="font-weight: bold;text-decoration: underline;">ET7108-81</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-p54nrb/NONO antibody (ET7108-81) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation