COMT Recombinant Rabbit Monoclonal Antibody [JG39-48]
Catalog# ET7108-89
COMT Recombinant Rabbit Monoclonal Antibody [JG39-48]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
COMT Recombinant Rabbit Monoclonal Antibody [JG39-48]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within COMT aa 11-107 / 271.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 30/24 kDa
Positive Control
HeLa cell lysate, MCF7 cell lysate, HepG2 cell lysate, rat testis tissue lysate, mouse testis tissue.
Conjugation
unconjugated
Clone Number
JG39-48
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:100
Target
Function
Catechol-O-methyltransferase (COMT) plays a crucial role in the regulation of central dopaminergic systems by catalyzing the inactivation of catechol-amines. It is widely distributed in most tissues in soluble and membrane-bound forms. COMT-mediated methylation metabolism of catecholamine neurotransmitters is a first-line detoxification pathway. A Val158Met polymorphism of the COMT gene affects activity of the enzyme and influences performance and efficiency of the prefrontal cortex of the brain. Sequential conversion of estradiol to methoxyestradiol by COMT, contributes to the antimitogenic effects of estradiol on vascular smooth muscle cell growth via estrogen receptor-independent mechanisms.
Background References
1. Chen J et al. Orientation and cellular distribution of membrane-bound catechol-O-methyltransferase in cortical neurons: implications for drug development. J Biol Chem 286:34752-34760 (2011).
2. Lee S-G et al. Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans. Hum Genet 116:319-328 (2005).
Sequence Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.
Tissue Specificity
Brain, liver, placenta, lymphocytes and erythrocytes.
Post-translational Modification
The N-terminus is blocked.
Subcellular Location
Cell membrane. Cytoplasm.
Synonyms
Catechol O methyltransferase antibody
Catechol O-methyltransferase antibody
COMT antibody
COMT_HUMAN antibody
EC 2.1.1.6 antibody
Images
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Western blot analysis of COMT on different lysates with Rabbit anti-COMT antibody (ET7108-89) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30/24 kDa
Observed band size: 30/24 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-89) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-COMT antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"