SUN1 Recombinant Rabbit Monoclonal Antibody [JG95-31]
Catalog# ET7108-91
SUN1 Recombinant Rabbit Monoclonal Antibody [JG95-31]
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WB
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IHC-P
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IF-Cell
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Human
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Rat
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unconjugated
Overview
Product Name
SUN1 Recombinant Rabbit Monoclonal Antibody [JG95-31]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SUN1 aa 90-240 / 812.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 87 kDa
Positive Control
A431 cell lysate, HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, rat kidney tissue lysates, HeLa, human breast tissue, human colon tissue.
Conjugation
unconjugated
Clone Number
JG95-31
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:50-1:200
Target
Function
UNC84A (UNC84 homolog A), also known as SUN1, is a multi-pass nuclear membrane protein that is involved in nuclear anchoring and migration. Highly expressed in heart, brain and testis, UNC84A functions as an A-type Lamin-binding protein that forms a link between the inner and outer nuclear envelope membranes. This link acts as a structural bridge between the nuclear interior and the Actin cytoskeleton and is essential for proper localization of nuclear envelope proteins. Additionally, UNC84A may be involved in telomere attachment and in normal testis development. UNC84A contains one UNC84 (SUN) domain and exists as four isoforms due to alternative splicing events.
Background References
1. Bupp J M et al. Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3. J Cell Biol 179:845-854 (2007).
2. Stewart-Hutchinson P J et al. Structural requirements for the assembly of LINC complexes and their function in cellular mechanical stiffness. Exp Cell Res 314:1892-1905 (2008).
Post-translational Modification
The disulfid bond with KASH domain containing nesprins is required for stability of the respective LINC complexes under tensile forces.
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Protein unc-84 homolog A antibody
Sad1 and UNC84 domain containing 1 antibody
Sad1/unc 84 protein like 1 antibody
Sad1/unc-84 protein-like 1 antibody
Sun domain-containing protein 1 antibody
Sun1 antibody
SUN1_HUMAN antibody
UNC 84 homolog A (C elegans) antibody
UNC 84A antibody
UNC84 A antibody
ExpandImages
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Western blot analysis of SUN1 on different lysates with Rabbit anti-SUN1 antibody (ET7108-91) at 1/2,000 dilution.
Lane 1: A431 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 90/100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-91) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling SUN1 with Rabbit anti-SUN1 antibody (ET7108-91) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SUN1 antibody (ET7108-91) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Western blot analysis of SUN1 on rat kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SUN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SUN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"