FKBP52 Recombinant Rabbit Monoclonal Antibody [JG96-30]
Catalog# ET7108-93
FKBP52 Recombinant Rabbit Monoclonal Antibody [JG96-30]
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
FKBP52 Recombinant Rabbit Monoclonal Antibody [JG96-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human FKBP52 aa 1-132 / 459.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 52 kDa
Positive Control
SK-Br-3 cell lysate, HeLa cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human prostate cancer tissue, 293T, MCF-7.
Conjugation
unconjugated
Clone Number
JG96-30
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:1,000
-
FC
-
1:1,000
Target
Function
Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.
Background References
1. Peattie D A et al. Expression and characterization of human FKBP52, an immunophilin that associates with the 90-kDa heat shock protein and is a component of steroid receptor complexes. Proc Natl Acad Sci USA 89:10974-10978 (1992).
2. Tai P-K K et al. Association of a 59-kilodalton immunophilin with the glucocorticoid receptor complex. Science 256:1315-1318 (1992).
Tissue Specificity
Widely expressed.
Post-translational Modification
Phosphorylation by CK2 results in loss of HSP90 binding activity.
Subcellular Location
Cytoplasm. Cytoskeleton. Microtubule. Mitochondrion. Nucleus.
Synonyms
51 kDa FK506-binding protein antibody
52 kDa FK506 binding protein antibody
52 kDa FK506-binding protein antibody
52 kDa FKBP antibody
59 kDa immunophilin antibody
FK506 binding protein 4 antibody
FK506-binding protein 4 antibody
FKBP 4 antibody
FKBP 52 antibody
FKBP 59 antibody
ExpandImages
-
Western blot analysis of FKBP52 on different lysates with Rabbit anti-FKBP52 antibody (ET7108-93) at 1/2,000 dilution.
Lane 1: SK-Br-3 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Mouse testis tissue lysate (40 µg/Lane)
Lane 3: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 52 kDa
Observed band size: 55 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-93) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-FKBP52 antibody (ET7108-93) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-93) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
ICC staining FKBP52 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining FKBP52 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Flow cytometric analysis of 293T cells labeling FKBP52.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET7108-93, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"