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Retinoid X Receptor alpha Recombinant Rabbit Monoclonal Antibody [JG99-38]

Usd: 385

Specification

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Catalog# ET7108-99

Retinoid X Receptor alpha Recombinant Rabbit Monoclonal Antibody [JG99-38]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Retinoid X Receptor alpha Recombinant Rabbit Monoclonal Antibody [JG99-38]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein corresponding to Human Retinoid X Receptor alpha aa 1-50 / 462.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 51 kDa

Positive Control

Jurkat cell lysate, A549 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, Mouse heart tissue lysate, Rat liver tissue lysate, Rat heart tissue lysate, human skin tissue, mouse liver tissue, rat liver tissue.

Conjugation

unconjugated

Clone Number

JG99-38

RRID

AB_3070890

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:400-1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes.

Background References

1. Gorla-Bajszczak A et al. Conserved amino acids in the ligand-binding and tau(i) domains of the peroxisome proliferator-activated receptor alpha are necessary for heterodimerization with RXR. Mol Cell Endocrinol 147:37-47 (1999).

2. Harish S et al. Serine 27, a human retinoid X receptor alpha residue, phosphorylated by protein kinase A is essential for cyclicAMP-mediated downregulation of RXRalpha function. Biochem Biophys Res Commun 279:853-857 (2000).

Sequence Similarity

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Tissue Specificity

Expressed in lung fibroblasts (at protein level). Expressed in monocytes. Highly expressed in liver, also found in kidney and brain.

Post-translational Modification

Acetylated by EP300; acetylation enhances DNA binding and transcriptional activity.; Phosphorylated on serine and threonine residues mainly in the N-terminal modulating domain (By similarity). Constitutively phosphorylated on Ser-21 in the presence or absence of ligand (By similarity). Under stress conditions, hyperphosphorylated by activated JNK on Ser-56, Ser-70, Thr-82 and Ser-260 (By similarity). Phosphorylated on Ser-27, in vitro, by PKA. This phosphorylation is required for repression of cAMP-mediated transcriptional activity of RARA.; Sumoylation negatively regulates transcriptional activity. Desumoylated specifically by SENP6.

Subcellular Location

Mitochondrion, Nucleus, Cytoplasm.

UNIPROT

SwissProt: P19793 Human

SwissProt: P28700 Mouse

SwissProt: Q05343 Rat

Synonyms

FLJ00280 antibody

FLJ00318 antibody

FLJ16020 antibody

FLJ16733 antibody

MGC102720 antibody

NR2B1 antibody

Nuclear receptor subfamily 2 group B member 1 antibody

OTTHUMP00000022510 antibody

Retinoic acid receptor RXR alpha antibody

Retinoic acid receptor RXR-alpha antibody

Expand

Images

  • Western blot analysis of Retinoid X Receptor alpha on different lysates with Rabbit anti-Retinoid X Receptor alpha antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate (low expression)<br />Lane 2: A549 cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Retinoid X Receptor alpha on different lysates with Rabbit anti-Retinoid X Receptor alpha antibody (ET7108-99) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate (low expression)
    Lane 2: A549 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 55 kDa

    Exposure time: 2 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-99) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Retinoid X Receptor alpha on different lysates with Rabbit anti-Retinoid X Receptor alpha antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/500 dilution.<br /><br />Lane 1: Human liver tissue lysate<br />Lane 2: Mouse liver tissue lysate<br />Lane 3: Mouse heart tissue lysate<br />Lane 4: Rat liver tissue lysate<br />Lane 5: Rat heart tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 51 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Retinoid X Receptor alpha on different lysates with Rabbit anti-Retinoid X Receptor alpha antibody (ET7108-99) at 1/500 dilution.

    Lane 1: Human liver tissue lysate
    Lane 2: Mouse liver tissue lysate
    Lane 3: Mouse heart tissue lysate
    Lane 4: Rat liver tissue lysate
    Lane 5: Rat heart tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 51 kDa
    Observed band size: 55 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-99) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Retinoid X Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Retinoid X Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-99, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody (ET7108-99) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-99" style="font-weight: bold;text-decoration: underline;">ET7108-99</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Retinoid X Receptor alpha antibody (ET7108-99) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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