NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63]
Catalog# ET7109-12
NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63]
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WB
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IF-Cell
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IHC-P
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IP
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Human
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unconjugated
Overview
Product Name
NXF1 Recombinant Rabbit Monoclonal Antibody [JE40-63]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human NXF1 aa 1-132 / 619.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, IP
Molecular Weight
Predicted band size: 70 kDa
Positive Control
293 cell lysates, SiHa cell lysates, HEK-293, human brain tissue, human colon tissue.
Conjugation
unconjugated
Clone Number
JE40-63
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:100
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IHC-P
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1:200
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IP
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1:10-1:50
Target
Function
The constitutive transport element (CTE) of type D retroviruses serves as a signal of nuclear export for unspliced viral RNAs. TAP (tip-associating protein, also known as NXF1) mediates the export of CTE-containing simian type D retroviral RNAs through binding directly to the CTE. TAP is associated with a recognized mRNA export pathway and is a member of the multigene family of NXF proteins. NXF proteins belong to an evolutionarily conserved family of proteins, which are characterized by a leucine-rich-repeat domain (LRR) followed by a region known as the nuclear transport factor 2 (NTF2)-like domain.
Background References
1. Grueter P et al. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol Cell 1:649-659 (1998).
2. Tang H et al. Specific interaction between RNA helicase A and Tap, two cellular proteins that bind to the constitutive transport element of type D retrovirus. J Biol Chem 275:32694-32700 (2000).
Sequence Similarity
Belongs to the NXF family.
Tissue Specificity
Expressed ubiquitously.
Subcellular Location
Nucleus. Cytoplasm.
UNIPROT
Synonyms
DKFZp667O0311 antibody
DmNXF1 antibody
MEX67 antibody
MEX67, yeast, homolog of antibody
Mex67p antibody
mRNA export factor TAP antibody
Mvb1 antibody
Nuclear RNA export factor 1 antibody
Nuclear RNA export factor 1 homolog (S. cerevisiae) antibody
nxf 1 antibody
ExpandImages
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Western blot analysis of NXF1 on 293 (1) and SiHa (2) cell lysate using anti-NXF1 antibody at 1/2,000 dilution.
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Immunocytochemistry analysis of HEK-293 cells labeling NXF1 with Rabbit anti-NXF1 antibody (ET7109-12) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NXF1 antibody (ET7109-12) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NXF1 antibody (ET7109-12) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-12) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-NXF1 antibody (ET7109-12) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-12) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"