Kininogen 1 Recombinant Rabbit Monoclonal Antibody [JE43-72]
Catalog# ET7109-34
Kininogen 1 Recombinant Rabbit Monoclonal Antibody [JE43-72]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
Kininogen 1 Recombinant Rabbit Monoclonal Antibody [JE43-72]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Kininogen 1 aa 594-644 / 644.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 72 kDa
Positive Control
Mouse stomach lysates, human liver tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
JE43-72
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor is a protein that in humans is encoded by the KNG1 gene. Kininogen-1 is the precursor protein to high-molecular-weight kininogen (HMWK), low-molecular-weight kininogen (LMWK), and bradykinin. The KNG1 gene uses alternative splicing to generate two different proteins: high-molecular-weight kininogen (HMWK) and low-molecular-weight kininogen (LMWK). HMWK in turn is cleaved by the enzyme kallikrein to produce bradykinin. HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Kininogen-1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.
Background References
1. Takagaki Y et al. Cloning and sequence analysis of cDNAs for human high molecular weight and low molecular weight prekininogens. Primary structures of two human prekininogens. J Biol Chem 260:8601-8609 (1985).
2. Kellermann J et al. Completion of the primary structure of human high-molecular-mass kininogen. The amino acid sequence of the entire heavy chain and evidence for its evolution by gene triplication. Eur J Biochem 154:471-478(1986).
Tissue Specificity
Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
Post-translational Modification
Bradykinin is released from kininogen by plasma kallikrein.; Hydroxylation of Pro-383 occurs prior to the release of bradykinin.; Phosphorylated by FAM20C in the extracellular medium.; N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Subcellular Location
Secreted.
UNIPROT
Synonyms
Alpha-2-thiol proteinase inhibitor antibody
BDK antibody
BK antibody
Bradykinin antibody
Bradykinin included antibody
Fitzgerald factor antibody
FLAUJEAC FACTOR antibody
High molecular weight kininogen antibody
HMWK antibody
Ile-Ser-Bradykinin antibody
ExpandImages
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Western blot analysis of Kininogen 1 on mouse stomach lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Kininogen 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-34) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Kininogen 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-34, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"