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AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]

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Specification

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Catalog# ET7109-45

AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

AP-A Recombinant Rabbit Monoclonal Antibody [JE46-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human AP-A aa 860-957 / 957.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 109 kDa

Positive Control

Human kidney tissue lysate, human kidney tissue, human small intestine tissue.

Conjugation

unconjugated

Clone Number

JE46-03

RRID

AB_3070931

Product Features

Form

Liquid

Concentration

1 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II. ENPEP is considered to be an essential and highly specific enzyme, which metabolizes and inactivates bioactive peptides such as angiotensin II (AngII) in the renin-angiotensin system. ENPEP is expressed in neoplastic lesions of the uterine cervix, and its levels are upregulated as the lesions progress from cervical intraepithelial neoplasms to invasive squamous cell carcinomas. ENPEP expression is absent in normal cells and is overexpressed in cancer cell lines compared with normal cells of mammary origin.

Background References

1. Yang Y et al. Structural insights into central hypertension regulation by human aminopeptidase A. J Biol Chem 288:25638-25645 (2013).

2. Ino K et al. Expression of aminopeptidase A in human gestational choriocarcinoma cell lines and tissues. Placenta 21:63-72 (2000).

Sequence Similarity

Belongs to the peptidase M1 family.

Tissue Specificity

Expressed in choriocarcinoma cancer cell lines (at protein level). Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.

Subcellular Location

Cell membrane, Membrane.

UNIPROT

SwissProt: Q07075 Human

Synonyms

Aminopeptidase A antibody

AMPE_HUMAN antibody

AP-A antibody

APA antibody

bp-1 antibody

bp1 antibody

CD249 antibody

Differentiation antigen gp160 antibody

EAP antibody

Enpep antibody

Expand

Images

  • Western blot analysis of AP-A on Human kidney tissue lysate with Rabbit anti-AP-A antibody (<a href="/products/ET7109-45" style="font-weight: bold;text-decoration: underline;">ET7109-45</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 2 minutes; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7109-45" style="font-weight: bold;text-decoration: underline;">ET7109-45</a>, 1/500 in 5% NFDM/TBST, 2 hours at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/30,000 in 5% NFDM/TBST, 1 hour at room temperature

    Western blot analysis of AP-A on Human kidney tissue lysate with Rabbit anti-AP-A antibody (ET7109-45) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 2 minutes; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7109-45, 1/500 in 5% NFDM/TBST, 2 hours at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/30,000 in 5% NFDM/TBST, 1 hour at room temperature

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AP-A antibody (<a href="/products/ET7109-45" style="font-weight: bold;text-decoration: underline;">ET7109-45</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-45" style="font-weight: bold;text-decoration: underline;">ET7109-45</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AP-A antibody (ET7109-45) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-45) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-AP-A antibody (<a href="/products/ET7109-45" style="font-weight: bold;text-decoration: underline;">ET7109-45</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-45" style="font-weight: bold;text-decoration: underline;">ET7109-45</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-AP-A antibody (ET7109-45) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"