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RNF40 Recombinant Rabbit Monoclonal Antibody [JE46-30]

Usd: 385

Specification

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Catalog# ET7109-46

RNF40 Recombinant Rabbit Monoclonal Antibody [JE46-30]

Application

  • WB

  • IP

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RNF40 Recombinant Rabbit Monoclonal Antibody [JE46-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human RNF40 aa 211-451 / 1,001.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP, IHC-P

Molecular Weight

Predicted band size: 114 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, MEF cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human colon tissue, mouse brain tissue.

Conjugation

unconjugated

Clone Number

JE46-30

RRID

AB_3070932

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IP

  • 1:10-1:50

  • IHC-P

  • 1:400

Target

Function

E3 ubiquitin-protein ligase BRE1B is an enzyme that in humans is encoded by the RNF40 gene. The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.

Background References

1. Zhu B et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. Mol Cell 20:601-611 (2005).

2. Kim J et al. RAD6-mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells. Cell 137:459-471 (2009).

Sequence Similarity

Belongs to the BRE1 family.

Tissue Specificity

Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O75150 Human

SwissProt: Q3U319 Mouse

SwissProt: Q8CJB9 Rat

Synonyms

95 kDa retinoblastoma associated protein antibody

95 kDa retinoblastoma protein binding protein antibody

95 kDa retinoblastoma-associated protein antibody

BRE 1B antibody

BRE1 B antibody

BRE1 E3 ubiquitin ligase homolog B antibody

BRE1-B antibody

BRE1B antibody

BRE1B_HUMAN antibody

DKFZp686K191 antibody

Expand

Images

  • Western blot analysis of RNF40 on different lysates with Rabbit anti-RNF40 antibody (<a href="/products/ET7109-46" style="font-weight: bold;text-decoration: underline;">ET7109-46</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: C2C12 cell lysate (20 µg/Lane)<br />Lane 4: MEF cell lysate (20 µg/Lane)<br />Lane 5: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 6: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 114 kDa<br />Observed band size: 130 kDa<br />Exposure time: 1 minute; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7109-46" style="font-weight: bold;text-decoration: underline;">ET7109-46</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RNF40 on different lysates with Rabbit anti-RNF40 antibody (ET7109-46) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: C2C12 cell lysate (20 µg/Lane)
    Lane 4: MEF cell lysate (20 µg/Lane)
    Lane 5: Mouse testis tissue lysate (40 µg/Lane)
    Lane 6: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 114 kDa
    Observed band size: 130 kDa
    Exposure time: 1 minute; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RNF40 antibody (<a href="/products/ET7109-46" style="font-weight: bold;text-decoration: underline;">ET7109-46</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-46" style="font-weight: bold;text-decoration: underline;">ET7109-46</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RNF40 antibody (ET7109-46) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-46) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RNF40 antibody (<a href="/products/ET7109-46" style="font-weight: bold;text-decoration: underline;">ET7109-46</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-46" style="font-weight: bold;text-decoration: underline;">ET7109-46</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RNF40 antibody (ET7109-46) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-46) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"