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NDUFAF1 Recombinant Rabbit Monoclonal Antibody [JE47-18]

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Specification

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Catalog# ET7109-54

NDUFAF1 Recombinant Rabbit Monoclonal Antibody [JE47-18]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NDUFAF1 Recombinant Rabbit Monoclonal Antibody [JE47-18]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human NDUFAF1 aa 1-190 / 327.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 38 kDa

Positive Control

K-562 cell lysate, HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, human kidney tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

JE47-18

RRID

AB_3070936

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. Part of the mitochondrial complex I assembly (MCIA) complex. The complex comprises at least TMEM126B, NDUFAF1, ECSIT, and ACAD9. Interacts with ECSIT. Interacts with ACAD9. At early stages of complex I assembly, it is found in intermediate subcomplexes that contain different subunits including NDUFB6, NDUFA6, NDUFA9, NDUFS3, NDUFS7, ND1, ND2 and ND3

Background References

1. Janssen R et al. CIA30 complex I assembly factor: a candidate for human complex I deficiency Hum. Genet. 110:264-270 (2002).

Sequence Similarity

Belongs to the CIA30 family.

Tissue Specificity

Ubiquitous.

Subcellular Location

Mitochondrion, Mitochondrion matrix.

UNIPROT

SwissProt: Q9Y375 Human

Synonyms

CGI 65 antibody

CGI65 antibody

CIA30 antibody

CIA30_HUMAN antibody

Complex I intermediate associated protein 30 antibody

Complex I intermediate associated protein 30 mitochondrial antibody

Complex I intermediate-associated protein 30, mitochondrial antibody

NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody

NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody

Expand

Images

  • Western blot analysis of NDUFAF1 on different lysates with Rabbit anti-NDUFAF1 antibody (<a href="/products/ET7109-54" style="font-weight: bold;text-decoration: underline;">ET7109-54</a>) at 1/2,000 dilution.<br /><br />Lane 1: K-562 cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: Jurkat cell lysate (20 µg/Lane)<br />Lane 4: HEK-293 cell lysate (20 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7109-54" style="font-weight: bold;text-decoration: underline;">ET7109-54</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NDUFAF1 on different lysates with Rabbit anti-NDUFAF1 antibody (ET7109-54) at 1/2,000 dilution.

    Lane 1: K-562 cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: Jurkat cell lysate (20 µg/Lane)
    Lane 4: HEK-293 cell lysate (20 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 33 kDa

    Exposure time: 25 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-54) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFAF1 antibody (<a href="/products/ET7109-54" style="font-weight: bold;text-decoration: underline;">ET7109-54</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-54" style="font-weight: bold;text-decoration: underline;">ET7109-54</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFAF1 antibody (ET7109-54) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NDUFAF1 antibody (<a href="/products/ET7109-54" style="font-weight: bold;text-decoration: underline;">ET7109-54</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-54" style="font-weight: bold;text-decoration: underline;">ET7109-54</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NDUFAF1 antibody (ET7109-54) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"