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BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]

Usd: 385

Specification

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Catalog# ET7109-62

BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human BAG2 aa 1-118 / 211.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 24 kDa

Positive Control

HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, A549 cell lysate, K562 cell lysate, Hela.

Conjugation

unconjugated

Clone Number

JE47-57

RRID

AB_3070942

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

Target

Function

BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.

Background References

1. Rauch JN et al. Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro. J. Biol. Chem. 289:1402-1414 (2014).

2. Takayama S et al. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. J. Biol. Chem. 274:781-786 (1999).

Subcellular Location

Cytoskeleton, Cytosol.

UNIPROT

SwissProt: O95816 Human

Synonyms

BAG 2 antibody

BAG family molecular chaperone regulator 2 antibody

BAG-2 antibody

BAG-family molecular chaperone regulator-2 antibody

Bag2 antibody

BAG2_HUMAN antibody

BCL 2 associated athanogene 2 antibody

Bcl-2-associated athanogene 2 antibody

BCL2 associated athanogene 2 antibody

BCL2-associated athanogene 2 antibody

Expand

Images

  • Western blot analysis of BAG2 on different lysates with Rabbit anti-BAG2 antibody (<a href="/products/ET7109-62" style="font-weight: bold;text-decoration: underline;">ET7109-62</a>) at 1/500 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: Hela cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: A549 cell lysate<br />Lane 5: K562 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 24 kDa<br />Observed band size: 24 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7109-62" style="font-weight: bold;text-decoration: underline;">ET7109-62</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of BAG2 on different lysates with Rabbit anti-BAG2 antibody (ET7109-62) at 1/500 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: A549 cell lysate
    Lane 5: K562 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 24 kDa
    Observed band size: 24 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-62) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7109-62" style="font-weight: bold;text-decoration: underline;">ET7109-62</a>, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-62, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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