BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]
Catalog# ET7109-62
BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]
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WB
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FC
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Human
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unconjugated
Overview
Product Name
BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human BAG2 aa 1-118 / 211.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
Hela cell lysate, Jurkat cell lysate, HEK-293 cell lysate, K-562 cell lysate, Hela.
Conjugation
unconjugated
Clone Number
JE47-57
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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FC
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1:50-1:100
Target
Function
BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Background References
1. Rauch JN et al. Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro. J. Biol. Chem. 289:1402-1414 (2014).
2. Takayama S et al. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. J. Biol. Chem. 274:781-786 (1999).
Subcellular Location
Cytoskeleton, Cytosol.
UNIPROT
Synonyms
BAG 2 antibody
BAG family molecular chaperone regulator 2 antibody
BAG-2 antibody
BAG-family molecular chaperone regulator-2 antibody
Bag2 antibody
BAG2_HUMAN antibody
BCL 2 associated athanogene 2 antibody
Bcl-2-associated athanogene 2 antibody
BCL2 associated athanogene 2 antibody
BCL2-associated athanogene 2 antibody
ExpandImages
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Western blot analysis of BAG2 on different lysates with Rabbit anti-BAG2 antibody (ET7109-62) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 46 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7109-62, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 23.8 kDa
Observed band size: 24 kDa -
Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-62, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"