Tryptophanyl tRNA synthetase / WRS Recombinant Rabbit Monoclonal Antibody [JE48-08]
Catalog# ET7109-73
Tryptophanyl tRNA synthetase / WRS Recombinant Rabbit Monoclonal Antibody [JE48-08]
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Tryptophanyl tRNA synthetase / WRS Recombinant Rabbit Monoclonal Antibody [JE48-08]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Tryptophanyl tRNA synthetase aa 385-471 / 471.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 53 kDa
Positive Control
K562 cell lysates, human lung tissue, A549.
Conjugation
unconjugated
Clone Number
JE48-08
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
Background References
1. Wakasugi K et al. A human aminoacyl-tRNA synthetase as a regulator of angiogenesis. Proc. Natl. Acad. Sci. U.S.A. 99:173-177 (2002).
2. Bange FC et al. An interferon-induced protein with release factor activity is a tryptophanyl-tRNA synthetase. FEBS Lett 300:162-166 (1992).
Sequence Similarity
Belongs to the class-I aminoacyl-tRNA synthetase family.
Post-translational Modification
Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
Gamma 2 antibody
GAMMA-2 antibody
Gamma2 antibody
hWRS antibody
IFI 53 antibody
IFI53 antibody
IFP 53 antibody
IFP53 antibody
Interferon induced protein 53 antibody
Interferon-induced protein 53 antibody
ExpandImages
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Western blot analysis of Tryptophanyl tRNA synthetase / WRS on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Tryptophanyl tRNA synthetase / WRS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-73, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"