APRIL/TNFSF13 Recombinant Rabbit Monoclonal Antibody [JE49-07]
Catalog# ET7109-89
APRIL/TNFSF13 Recombinant Rabbit Monoclonal Antibody [JE49-07]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
APRIL/TNFSF13 Recombinant Rabbit Monoclonal Antibody [JE49-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human APRIL / TNFSF13 aa 141-250 / 250.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 27 kDa
Positive Control
Hela cell and A549 cell lysates, A549.
Conjugation
unconjugated
Clone Number
JE49-07
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-Cell
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1:50
Target
Function
A proliferation-inducing ligand (APRIL), also known as tumor necrosis factor ligand superfamily member 13 (TNFSF13), is a protein of the TNF superfamily recognized by the cell surface receptor TACI. The protein encoded by this gene is a member of the tumor necrosis factor ligand (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in APRIL have normal immune system development. However, APRIL-deficient mice have also been reported to possess a reduced ability to support plasma cell survival. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Background References
1. Yu G. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat. Immunol. 1:252-256 (2000).
Sequence Similarity
Belongs to the tumor necrosis factor family.
Tissue Specificity
Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
Post-translational Modification
The precursor is cleaved by furin.
Subcellular Location
Secreted.
UNIPROT
Synonyms
A proliferation inducing ligand antibody
A proliferation-inducing ligand antibody
APRIL antibody
CD256 antibody
TALL-2 antibody
TALL2 antibody
TNF related death ligand antibody
TNF- and APOL-related leukocyte expressed ligand 2 antibody
TNF-related death ligand 1 antibody
TNF13_HUMAN antibody
ExpandImages
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Western blot analysis of APRIL/TNFSF13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Predicted band size: 27 kDa
Observed band size: 14 kDa -
ICC staining of APRIL/TNFSF13 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"