CD239 Recombinant Rabbit Monoclonal Antibody [JE51-07]
Catalog# ET7110-04
CD239 Recombinant Rabbit Monoclonal Antibody [JE51-07]
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
CD239 Recombinant Rabbit Monoclonal Antibody [JE51-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD239 aa 579-628 / 628.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 67 kDa
Positive Control
A431 cell lysate, human kidney tissue, human placenta tissue, JAR.
Conjugation
unconjugated
Clone Number
JE51-07
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
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FC
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1:50-1:100
Target
Function
Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membrane glycoprotein that in humans is encoded by the BCAM gene. BCAM has also recently been designated CD239 (cluster of differentiation 239). Lutheran blood group glycoprotein is a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five, N-terminus, extracellular immunoglobulin domains, a single transmembrane domain, and a short, C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. BCAM has been shown to interact with Laminin, alpha 5.
Background References
1. Hussein K. et. al. Expression of adhesion factor CD239 in bone marrow cells in chronic myeloproliferative diseases. J Thromb Thrombolysis. 2009 Oct;28(3):299-303.
2. Kikkawa Y. et. al. Internalization of CD239 highly expressed in breast cancer cells: a potential antigen for antibody-drug conjugates. Sci Rep. 2018 Apr 26;8(1):6612.
Tissue Specificity
Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.
Post-translational Modification
Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.
Subcellular Location
Membrane.
UNIPROT
Synonyms
Antigen identified by monoclonal antibody F8 antibody
AU antibody
Auberger B antigen antibody
B CAM cell surface glycoprotein antibody
B cell adhesion molecule antibody
B-CAM cell surface glycoprotein antibody
Basal cell adhesion molecule (Lu and Au blood groups) antibody
Basal cell adhesion molecule (Lutheran blood group) antibody
Basal cell adhesion molecule antibody
Basal cell adhesion molecule Lu and Au blood groups antibody
ExpandImages
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☑ Knockdown (KD)
Western blot analysis of CD239 on different lysates with Rabbit anti-CD239 antibody (ET7110-04) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-CD239 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 67 kDa
Observed band size: 85 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-04) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of CD239 on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD239 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-04, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD239 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-04, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of CD239 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-04, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"