Phospho-HER3/ErbB3 (Y1222) Recombinant Rabbit Monoclonal Antibody [JE51-89]
Catalog# ET7110-10
Phospho-HER3/ErbB3 (Y1222) Recombinant Rabbit Monoclonal Antibody [JE51-89]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Phospho-HER3/ErbB3 (Y1222) Recombinant Rabbit Monoclonal Antibody [JE51-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to a region surrounding Tyrosine 1222 of Human ErbB3.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 148 kDa
Positive Control
MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, human thyroid tissue, human skin tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
JE51-89
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
Target
Function
The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.
Background References
1. Sağsöz H. et. al. Expression and localisation of epidermal growth factor receptors and their ligands in the lower genital tract of cycling cows. Reprod Fertil Dev. 2019 Jul 4.
2. Dietrich M. et. al. Protein kinase C regulates ErbB3 turnover. Exp Cell Res. 2019 Jun 21.
Sequence Similarity
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Tissue Specificity
Epithelial tissues and brain.
Post-translational Modification
Autophosphorylated. Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
Subcellular Location
Cell membrane, secreted.
UNIPROT
Synonyms
c erbB 3 antibody
c erbB3 antibody
Erb b2 receptor tyrosine kinase 3 antibody
ErbB 3 antibody
ERBB3 antibody
ERBB3 protein antibody
erbB3 S antibody
ERBB3_HUMAN antibody
Glial growth factor receptor antibody
HER 3 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-HER3/ErbB3 (Y1222) on different lysates with Rabbit anti-Phospho-HER3/ErbB3 (Y1222) antibody (ET7110-10) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate
Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 148 kDa
Observed band size: 180 kDa
Exposure time: 2 minutes 37 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-Phospho-HER3/ErbB3 (Y1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Phospho-HER3/ErbB3 (Y1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Phospho-HER3/ErbB3 (Y1222) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-10, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"