WRN Recombinant Rabbit Monoclonal Antibody [JE52-72]
Catalog# ET7110-26
WRN Recombinant Rabbit Monoclonal Antibody [JE52-72]
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WB
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Human
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unconjugated
Overview
Product Name
WRN Recombinant Rabbit Monoclonal Antibody [JE52-72]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human WRN aa 1,260-1,432 / 1,432.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 162 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Clone Number
JE52-72
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand break repair after gamma-irradiation.
Background References
1. Lebel M. et. al. Werner syndrome (WRN) gene variants and their association with altered function and age-associated diseases. Ageing Res Rev. 2018 Jan;41:82-97.
2. Orlovetskie N. et. al. Targeted inhibition of WRN helicase, replication stress and cancer. Biochim Biophys Acta Rev Cancer. 2017 Jan;1867(1):42-48.
Sequence Similarity
Belongs to the helicase family. RecQ subfamily.
Post-translational Modification
Phosphorylated by PRKDC.
Subcellular Location
Nucleolus, nucleus, nucleoplasm.
UNIPROT
Synonyms
DKFZp686C2056 antibody
DNA helicase antibody
DNA helicase, RecQ like type 3 antibody
Exonuclease WRN antibody
HGNC 12791 antibody
OTTHUMP00000225301 antibody
RecQ protein-like 2 antibody
RecQ-like type 3 antibody
RecQ3 antibody
RECQL2 antibody
ExpandImages
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Western blot analysis of WRN on different lysates with Rabbit anti-WRN antibody (ET7110-26) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K-562 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 162 kDa
Observed band size: 162 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Species: Human
Cell sample: HeLa
Antibody concentration: 1: 1000
Date by conrtesy of: Mr. Haihua Xie, School of Basic Medical Sicences, Zhejiang University
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"