DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]
Catalog# ET7110-31
DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]
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WB
-
FC
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Human
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Mouse
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unconjugated
Overview
Product Name
DBF4 Recombinant Rabbit Monoclonal Antibody [JE52-50]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human DBF4 aa 1-107 / 674.
Species Reactivity
Human, Mouse
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 77 kDa
Positive Control
293T cell lysate, Hela cell lysate, SW480 cell lysate, Jurkat cell lysate, F9.
Conjugation
unconjugated
Clone Number
JE52-50
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
Target
Function
The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.
Background References
1. Cheng AN. et. al. Cdc7-Dbf4-mediated phosphorylation of HSP90-S164 stabilizes HSP90-HCLK2-MRN complex to enhance ATR/ATM signaling that overcomes replication stress in cancer. Sci Rep. 2017 Dec 5;7(1):17024.
2. Bruck I. et. al. The Dbf4-Cdc7 kinase promotes Mcm2-7 ring opening to allow for single-stranded DNA extrusion and helicase assembly. J Biol Chem. 2017 Jun 16;292(24):10320.
Tissue Specificity
Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Post-translational Modification
Phosphorylation increases its interaction with PSIP1.
Subcellular Location
Nucleus.
Synonyms
Activator of S phase kinase antibody
ASK antibody
CHIF antibody
Chiffon homolog A antibody
DBF4 antibody
DBF4 homolog (S. cerevisiae) antibody
DBF4 homolog antibody
DBF4 type zinc finger containing protein 1 antibody
DBF4 zinc finger A antibody
DBF4 zinc finger antibody
ExpandImages
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Western blot analysis of DBF4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: SW480 cell lysate
Lane 4: Jurkat cell lysate -
Flow cytometric analysis of DBF4 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"