CDC123 Recombinant Rabbit Monoclonal Antibody [JE53-14]
Catalog# ET7110-32
CDC123 Recombinant Rabbit Monoclonal Antibody [JE53-14]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
CDC123 Recombinant Rabbit Monoclonal Antibody [JE53-14]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CDC123 aa 1-120 / 336.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 39 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, 293T cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE53-14
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
The eukaryotic cell division cycle consists of a number of gene-controlled sequences that involve cyclin dependent kinases (Cdks) and cell division control (Cdc) proteins. Cdc123 (Cell division cycle protein 123), also known as D123, is a 336 amino acid cytoplasmic protein that is involved in cell cycle control. Widely expressed with high expression in thymus, spleen, ovary, testis, small intestine and leukocytes, Cdc123 functions to destabilize Chfr (checkpoint with forkhead and ring finger domain) proteins which, when accumulated, block the G to S phase transition. Cdc123 prevents the Chfr proteins from collecting in the cell, thereby allowing the cell to enter the S phase. Due to its role in cell cycle control, Cdc123 is thought to be a basal marker for luminal breast cancers.
Background References
1. Tarnowski M. et. al. CDC123/CAMK1D gene rs12779790 polymorphism and rs10811661 polymorphism upstream of the CDKN2A/2B gene in women with gestational diabetes. J Perinatol. 2017 Apr;37(4):345-348.
2. Burroughs AM. et. al. The eukaryotic translation initiation regulator CDC123 defines a divergent clade of ATP-grasp enzymes with a predicted role in novel protein modifications. Biol Direct. 2015 May 15;10:21.
Sequence Similarity
Belongs to the CDC123 family.
Tissue Specificity
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes with the highest expression in testis.
Subcellular Location
Cytoplasm.
Synonyms
C10orf7 antibody
CD123_HUMAN antibody
CDC123 antibody
Cell division cycle 123 antibody
Cell division cycle 123 homolog antibody
Cell division cycle protein 123 homolog antibody
D123 antibody
HT 1080 antibody
HT-1080 antibody
Protein D123 antibody
ExpandImages
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Western blot analysis of CDC123 on different lysates with Rabbit anti-CDC123 antibody (ET7110-32) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: 293T cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 45 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-32) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"