WNT5B Recombinant Rabbit Monoclonal Antibody [JE53-67]
Catalog# ET7110-34
WNT5B Recombinant Rabbit Monoclonal Antibody [JE53-67]
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WB
-
FC
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Human
-
unconjugated
Overview
Product Name
WNT5B Recombinant Rabbit Monoclonal Antibody [JE53-67]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human WNT5B aa 260-359 / 359.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, LOVO.
Conjugation
unconjugated
Clone Number
JE53-67
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
FC
-
1:50-1:100
Target
Function
Protein Wnt-5b is a protein that in humans is encoded by the WNT5B gene. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.
Background References
1. Zhang Q. et. al. WNT5B exerts oncogenic effects and is negatively regulated by miR-5587-3p in lung adenocarcinoma progression. Oncogene. 2019 Oct 30.
2. Jiang S. et. al. WNT5B governs the phenotype of basal-like breast cancer by activating WNT signaling. Cell Commun Signal. 2019 Aug 28;17(1):109.
Sequence Similarity
Belongs to the Wnt family.
Post-translational Modification
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Subcellular Location
Extracellular matrix, Secreted.
UNIPROT
Synonyms
MGC2648 antibody
Protein Wnt-5b antibody
Wingless type MMTV integration site family, member 5B antibody
WNT 5B antibody
WNT 5B protein antibody
Wnt family member 5B antibody
Wnt5b antibody
WNT5B protein antibody
WNT5B_HUMAN antibody
Images
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Western blot analysis of WNT5B on different lysates with Rabbit anti-WNT5B antibody (ET7110-34) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7110-34, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 40 kDa
Observed band size: 45 kDa -
Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"