ECM1 Recombinant Rabbit Monoclonal Antibody [JE54-17]
Catalog# ET7110-41
ECM1 Recombinant Rabbit Monoclonal Antibody [JE54-17]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
ECM1 Recombinant Rabbit Monoclonal Antibody [JE54-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ECM1 aa 81-232 / 540.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 61 kDa
Positive Control
THP-1 cell lysate, Rat heart tissue lysate, A431 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE54-17
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
ECM1 (extracellular matrix protein 1), also known as secretory component p85, is a secreted glycoprotein that is essential for the proper structure and function of the skin. It is widely expressed and localizes to the extracellular matrix. ECM1 binds to a variety of extracellular matrix components, including Perlecan, Fibulin and matrix metalloproteinase-9 (MMP-9), and participates in the structural organization of the dermis. In addition, ECM1 enhances the association of Collagen Type IV with Laminin 332 suggesting that it is a key player in interstitial dermis and the dermal-epidermal junction. Mutations in the gene encoding ECM1 result in the autosomal recessive disorder lipoid proteinosis (LiP). LiP is characterized by hyalinization of the dermis and reduplication of the basement membrane of the skin. LiP patients exhibit thickening of the skin and mucosae. Four splice variants (known as ECM1a-ECM1d) exist for ECM1.
Background References
1. Fan W. et. al. ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice. Gastroenterology. 2019 Nov;157(5):1352-1367.e13.
2. Gómez-Contreras P. et. al. Extracellular matrix 1 (ECM1) regulates the actin cytoskeletal architecture of aggressive breast cancer cells in part via S100A4 and Rho-family GTPases. Clin Exp Metastasis. 2017 Jan;34(1):37-49.
Tissue Specificity
Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin; wide expression is observed throughout the dermis with minimal expression in the epidermis.
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Synonyms
ECM 1 antibody
Ecm1 antibody
ECM1_HUMAN antibody
Extracellular matrix protein 1 antibody
Secretory component p85 antibody
URBWD antibody
Images
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Western blot analysis of ECM1 on different lysates with Rabbit anti-ECM1 antibody (ET7110-41) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: Rat heart tissue lysate
Lane 3: A431 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: Lane 1-2: 14 seconds; Lane 3-4: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-41) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"