ABI2 Recombinant Rabbit Monoclonal Antibody [JE53-89]
Catalog# ET7110-42
ABI2 Recombinant Rabbit Monoclonal Antibody [JE53-89]
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ABI2 Recombinant Rabbit Monoclonal Antibody [JE53-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ABI2 aa 312-411 / 513.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 56 kDa
Positive Control
K-562 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, PC-12.
Conjugation
unconjugated
Clone Number
JE53-89
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation. Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge. Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells.
Background References
1. Wang H. et. al. Abscisic Acid Signaling Inhibits Brassinosteroid Signaling through Dampening the Dephosphorylation of BIN2 by ABI1 and ABI2. Mol Plant. 2018 Feb 5;11(2):315-325.
2. Chen J. et. al. FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):E5519-27.
Sequence Similarity
Belongs to the ABI family.
Tissue Specificity
Widely expressed. Abundant in testes, ovary, thymus, and colon, with lower but detectable levels in prostate, peripheral blood leukocytes, and spleen.
Post-translational Modification
Phosphorylated by ABL1.
Subcellular Location
Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Nucleus.
Synonyms
Abelson interactor 2 antibody
ABI 2 antibody
Abi-2 antibody
ABI2 antibody
ABI2_HUMAN antibody
ABI2B antibody
Abl binding protein 3 antibody
Abl interacting protein 1 (SH3 containing protein) antibody
Abl interactor 2 antibody
Abl interactor protein 2b antibody
ExpandImages
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Western blot analysis of ABI2 on different lysates with Rabbit anti-ABI2 antibody (ET7110-42) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 73 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of PC-12 cells labeling ABI2 with Rabbit anti-ABI2 antibody (ET7110-42) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ABI2 antibody (ET7110-42) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of PC-12 cells labeling ABI2.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET7110-42, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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