Neurocan Recombinant Rabbit Monoclonal Antibody [JE54-15]
Catalog# ET7110-59
Neurocan Recombinant Rabbit Monoclonal Antibody [JE54-15]
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IHC-P
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Human
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unconjugated
Overview
Product Name
Neurocan Recombinant Rabbit Monoclonal Antibody [JE54-15]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Neurocan aa 1-96 / 1,321.
Species Reactivity
Human
Validated Applications
IHC-P
Molecular Weight
Predicted band size 143 kDa
Positive Control
Human brain tissue.
Conjugation
unconjugated
Clone Number
JE54-15
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:1,000
Target
Function
Neurocan core protein is a protein that in humans is encoded by the NCAN gene. Neurocan is a member of the lectican / chondroitin sulfate proteoglycan protein families and consists of neurocan core protein and chondroitin sulfate. It is thought to be involved in the modulation of cell adhesion and migration. Neurocan is a significant component of the extracellular matrix, and its levels are modulated by a variety of factors, but mice in which the NCAN gene has been knocked out show no easily observable defects in brain development or behavior. However, a genome-wide association study published in 2011 identified Neurocan as a susceptibility factor for bipolar disorder. A more comprehensive study published in 2012 confirmed that association. The 2012 study examined correlations between NCAN alleles and various symptoms of bipolar disorder, and also examined the behavior of NCAN knockout mice. In the human subjects, it was found that NCAN genotype was strongly associated with manic symptoms but not with depressive symptoms. In the mice, the absence of functional Neurocan resulted in a variety of manic-like behaviors, which could be normalized by administering lithium.
Background References
1. Fontanil T. et. al. Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies. Cell Physiol Biochem. 2019;52(5):1003-1016.
2. Mohan V. et. al. Neurocan Inhibits Semaphorin 3F Induced Dendritic Spine Remodeling Through NrCAM in Cortical Neurons. Front Cell Neurosci. 2018 Oct 9;12:346.
Sequence Similarity
Belongs to the aggrecan/versican proteoglycan family.
Tissue Specificity
Brain.
Post-translational Modification
O-glycosylated.
Subcellular Location
Secreted.
UNIPROT
Synonyms
Chondroitin sulfate proteoglycan 3 antibody
Cspg3 antibody
Ncan antibody
NCAN_HUMAN antibody
NEUR antibody
Neurocan core protein antibody
Neurocan proteoglycan antibody
Images
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Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Neurocan antibody (ET7110-59) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"