Syntaxin 1a Recombinant Rabbit Monoclonal Antibody [JE54-37]
Catalog# ET7110-68
Syntaxin 1a Recombinant Rabbit Monoclonal Antibody [JE54-37]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Syntaxin 1a Recombinant Rabbit Monoclonal Antibody [JE54-37]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Syntaxin 1a aa 1-50 / 288.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Molecular Weight
Predicted band size: 33 kDa
Positive Control
Mouse bone marrow tissue lysates, human cerebellum tissue lysates, rat brain tissue, human kidney tissue, human pancreas tissue, SH-SY5Y.
Conjugation
unconjugated
Clone Number
JE54-37
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Syntaxin-1A is a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. Syntaxin-1A is a key protein in ion channel regulation and synaptic exocytosis.
Background References
1. Petro CD. et. al. Shiga Toxin Type 1a (Stx1a) Reduces the Toxicity of the More Potent Stx2a In Vivo and In Vitro. Infect Immun. 2019 Mar.
2. Wang M. et. al. STX1A gene variations contribute to the susceptibility of children attention-deficit/hyperactivity disorder: a case-control association study.Eur Arch Psychiatry Clin Neurosci. 2019 Sep.
Sequence Similarity
Belongs to the syntaxin family.
Tissue Specificity
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
Post-translational Modification
Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.; Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis.
Subcellular Location
Cell membrane, synaptic vesicle membrane, synaptosome; Secreted.
Synonyms
HPC 1 antibody
Neuron specific antigen HPC1 antibody
Neuron-specific antigen HPC-1 antibody
OTTHUMP00000174615 antibody
OTTHUMP00000174616 antibody
OTTHUMP00000174617 antibody
OTTHUMP00000174618 antibody
P35-1 antibody
STX1 antibody
STX1A antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-68) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-68) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-68) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Syntaxin 1a was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-68, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Western blot analysis of Syntaxin 1a on different lysates with Rabbit anti-Syntaxin 1a antibody (ET7110-68) at 1/1,000 dilution.
Lane 1: Rat cerebellum tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Exposure time: 30 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7110-68, 1/1,000 2 hour at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 33.0 kDa
Observed band size: 37 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"