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TNFRSF19 Recombinant Rabbit Monoclonal Antibody [JE54-51]

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Specification

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Catalog# ET7110-74

TNFRSF19 Recombinant Rabbit Monoclonal Antibody [JE54-51]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TNFRSF19 Recombinant Rabbit Monoclonal Antibody [JE54-51]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TNFRSF19 aa 80-220 / 423.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 46 kDa

Positive Control

Mouse testis tissue lysate, rat large intestine tissue lysate, A431 cell lysate, human prostate carcinoma tissue, human small intestine tissue, HepG2.

Conjugation

unconjugated

Clone Number

JE54-51

RRID

AB_3071036

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY is a human gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TNF receptor associated factor (TRAF) family members, and to activate c-Jun N-terminal kinases (JNK) signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Background References

1. Deng C. et. al. TNFRSF19 Inhibits TGFβ Signaling through Interaction with TGFβ Receptor Type I to Promote Tumorigenesis. Cancer Res. 2018 Jul.

2. Shao L. et. al. The inherited variations of a p53-responsive enhancer in 13q12.12 confer lung cancer risk by attenuating TNFRSF19 expression. Genome Biol. 2019 May.

Tissue Specificity

Highly expressed in prostate. Detected at lower levels in thymus, spleen, testis, uterus, small intestine, colon and peripheral blood leukocytes.

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q9NS68 Human

SwissProt: Q9JLL3 Mouse

Synonyms

TAJ alpha antibody

TAJ antibody

TNFRSF 19 antibody

Toxicity and JNK inducer antibody

TRADE antibody

TROY antibody

Tumor necrosis factor receptor superfamily member 19 antibody

Images

  • Western blot analysis of TNFRSF19 on different lysates with Rabbit anti-TNFRSF19 antibody (<a href="/products/ET7110-74" style="font-weight: bold;text-decoration: underline;">ET7110-74</a>) at 1/500 dilution.<br /><br />Lane 1: mouse testis tissue lysate(20 µg/Lane)<br />Lane 2: rat large intestine tissue lysate (20 µg/Lane)<br />Lane 3: A431 cell lysate (10 µg/Lane)<br /><br />Predicted band size: 46 kDa<br />Observed band size: 46 kDa<br /><br />Exposure time: 1 minutes;<br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-74" style="font-weight: bold;text-decoration: underline;">ET7110-74</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TNFRSF19 on different lysates with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/500 dilution.

    Lane 1: mouse testis tissue lysate(20 µg/Lane)
    Lane 2: rat large intestine tissue lysate (20 µg/Lane)
    Lane 3: A431 cell lysate (10 µg/Lane)

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

    Exposure time: 1 minutes;
    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-74) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-TNFRSF19 antibody (<a href="/products/ET7110-74" style="font-weight: bold;text-decoration: underline;">ET7110-74</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-74" style="font-weight: bold;text-decoration: underline;">ET7110-74</a>) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-74) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-TNFRSF19 antibody (<a href="/products/ET7110-74" style="font-weight: bold;text-decoration: underline;">ET7110-74</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-74" style="font-weight: bold;text-decoration: underline;">ET7110-74</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-TNFRSF19 antibody (ET7110-74) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-74) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of TNFRSF19 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET7110-74" style="font-weight: bold;text-decoration: underline;">ET7110-74</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of TNFRSF19 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation