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Alpha SNAP Recombinant Rabbit Monoclonal Antibody [JE54-66]

Usd: 385

Specification

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Catalog# ET7110-79

Alpha SNAP Recombinant Rabbit Monoclonal Antibody [JE54-66]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Alpha SNAP Recombinant Rabbit Monoclonal Antibody [JE54-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Alpha SNAP aa 161-295 / 295.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 33 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, Rat liver tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE54-66

RRID

AB_3071040

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene. The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37%, 60%, and 67% identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP (this protein), gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types.

Background References

1. de Paola M. et. al. Pleiotropic effects of alpha-SNAP M105I mutation on oocyte biology: ultrastructural and cellular changes that adversely affect female fertility in mice. Sci Rep. 2019 Nov

2. Datta SP. et. al. Multiple paralogues of α-SNAP in Giardia lamblia exhibit independent subcellular localization and redistribution during encystation and stress. Parasit Vectors. 2018 Oct

Sequence Similarity

Belongs to the SNAP family.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P54920 Human

SwissProt: Q9DB05 Mouse

SwissProt: P54921 Rat

Synonyms

Alpha soluble NSF attachment protein antibody

Alpha-soluble NSF attachment protein antibody

N ethylmaleimide sensitive factor attachment protein alpha antibody

N-ethylmaleimide-sensitive factor attachment protein alpha antibody

napA antibody

NSF attachment protein alpha antibody

SNAA_HUMAN antibody

SNAP alpha antibody

SNAP-alpha antibody

SNAPA antibody

Images

  • Western blot analysis of Alpha SNAP on different lysates with Rabbit anti-Alpha SNAP antibody (<a href="/products/ET7110-79" style="font-weight: bold;text-decoration: underline;">ET7110-79</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 4: Rat liver tissue lysate (40 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 6: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 33 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-79" style="font-weight: bold;text-decoration: underline;">ET7110-79</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Alpha SNAP on different lysates with Rabbit anti-Alpha SNAP antibody (ET7110-79) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 4: Rat liver tissue lysate (40 µg/Lane)
    Lane 5: Mouse brain tissue lysate (40 µg/Lane)
    Lane 6: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 33 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-79) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"