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PEX19 Recombinant Rabbit Monoclonal Antibody [JE54-93]

Usd: 385

Specification

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Catalog# ET7110-89

PEX19 Recombinant Rabbit Monoclonal Antibody [JE54-93]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PEX19 Recombinant Rabbit Monoclonal Antibody [JE54-93]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human PEX19 aa 1-140 / 299.

Species Reactivity

Human, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size 33 kDa

Positive Control

Raji cell lysate, Jurkat cell lysate, HeLa cell lysate, C6 cell lysate, Rat liver tissue lysate, rat liver tissue, rat small intestine tissue.

Conjugation

unconjugated

Clone Number

JE54-93

RRID

AB_3071048

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. These disorders have at least 14 complementation groups, with more than one phenotype being observed for some complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14), which is also known as PBD-CGJ. Alternative splicing results in multiple transcript variants.

Background References

1. Cichocki BA. et. al. Pex19 is involved in importing dually targeted tail-anchored proteins to both mitochondria and peroxisomes. Traffic. 2018 Oct.

2. Jansen RLM. et. al. The peroxisome biogenesis factors Pex3 and Pex19: multitasking proteins with disputed functions. FEBS Lett. 2019 Mar.

Sequence Similarity

Belongs to the peroxin-19 family.

Tissue Specificity

Ubiquitously expressed. Isoform 1 is strongly predominant in all tissues except in utero where isoform 2 is the main form.

Subcellular Location

Peroxisome membrane, cytoplasmic side, cytoplasm.

UNIPROT

SwissProt: P40855 Human

SwissProt: Q9QYU1 Rat

Synonyms

33 kDa housekeeping protein antibody

D1S2223E antibody

HK33 antibody

Housekeeping gene 33kD antibody

OK/SW-cl.22 antibody

PBD12A antibody

Peroxin 19 antibody

Peroxin-19 antibody

Peroxisomal biogenesis factor 19 antibody

Peroxisomal farnesylated protein antibody

Expand

Images

  • Western blot analysis of PEX19 on different lysates with Rabbit anti-PEX19 antibody (<a href="/products/ET7110-89" style="font-weight: bold;text-decoration: underline;">ET7110-89</a>) at 1/5,000 dilution.<br /><br />Lane 1: Raji cell lysate (20 µg/Lane)<br />Lane 2: Jurkat cell lysate (20 µg/Lane)<br />Lane 3: HeLa cell lysate (20 µg/Lane)<br />Lane 4: C6 cell lysate (20 µg/Lane)<br />Lane 5: Rat liver tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 33 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 46 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7110-89" style="font-weight: bold;text-decoration: underline;">ET7110-89</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PEX19 on different lysates with Rabbit anti-PEX19 antibody (ET7110-89) at 1/5,000 dilution.

    Lane 1: Raji cell lysate (20 µg/Lane)
    Lane 2: Jurkat cell lysate (20 µg/Lane)
    Lane 3: HeLa cell lysate (20 µg/Lane)
    Lane 4: C6 cell lysate (20 µg/Lane)
    Lane 5: Rat liver tissue lysate (30 µg/Lane)

    Predicted band size: 33 kDa
    Observed band size: 40 kDa

    Exposure time: 46 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-89) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PEX19 antibody (<a href="/products/ET7110-89" style="font-weight: bold;text-decoration: underline;">ET7110-89</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-89" style="font-weight: bold;text-decoration: underline;">ET7110-89</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-PEX19 antibody (ET7110-89) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-89) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-PEX19 antibody (<a href="/products/ET7110-89" style="font-weight: bold;text-decoration: underline;">ET7110-89</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-89" style="font-weight: bold;text-decoration: underline;">ET7110-89</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-PEX19 antibody (ET7110-89) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-89) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation