Skip to content

RanBP9 Recombinant Rabbit Monoclonal Antibody [JE55-10]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET7110-95

RanBP9 Recombinant Rabbit Monoclonal Antibody [JE55-10]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RanBP9 Recombinant Rabbit Monoclonal Antibody [JE55-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human RANBP9 aa 90-205 / 1,041.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 116 kDa

Positive Control

Mouse hippocampus tissue lysate, rat kidney tissue lysate, SK-Br-3 cell lysate, rat testis tissue, human kidney tissue, mouse heart tissue.

Conjugation

unconjugated

Clone Number

JE55-10

RRID

AB_3071054

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

Target

Function

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS7, RPL18A, RPL6, histone H2A, histone H2B and histone. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. Mediates the nuclear import of actin .

Background References

1. Qin C. et. al. RANBP9 suppresses tumor proliferation in colorectal cancer. Oncol Lett. 2019 May.

2. Woo JA. et. al. Enhanced tau pathology via RanBP9 and Hsp90/Hsc70 chaperone complexes. Hum Mol Genet. 2017 Oct.

Sequence Similarity

Belongs to the importin beta family.

Subcellular Location

Nucleus, cytoplasm.

UNIPROT

SwissProt: Q96P70 Human

SwissProt: Q91YE6 Mouse

Entrez Gene: 304817 Rat

Synonyms

Imp9 antibody

Imp9a antibody

Imp9b antibody

Importin-9 antibody

Importin-9a antibody

Ipo9 antibody

IPO9_HUMAN antibody

Ran-binding protein 9 antibody

RanBP9 antibody

Images

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RanBP9 antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RanBP9 antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-RanBP9 antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-95) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/1,000 dilution.<br /><br />Lane 1: C2C12 (Mouse myoblasts) cell lysate <br />Lane 2: Mouse testis tissue lysate <br />Lane 3: Rat testis tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 116.0 kDa<br />Observed band size: 110 kDa

    Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/1,000 dilution.

    Lane 1: C2C12 (Mouse myoblasts) cell lysate
    Lane 2: Mouse testis tissue lysate
    Lane 3: Rat testis tissue lysate

    Lysates/proteins at 40 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7110-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 116.0 kDa
    Observed band size: 110 kDa

  • Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (<a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat (Human acute T-cell leukemia cells) cell lysate <br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7110-95" style="font-weight: bold;text-decoration: underline;">ET7110-95</a>, 1/1,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 116.0 kDa<br />Observed band size: 110 kDa

    Western blot analysis of RanBP9 on different lysates with Rabbit anti-RanBP9 antibody (ET7110-95) at 1/1,000 dilution.

    Lane 1: Jurkat (Human acute T-cell leukemia cells) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7110-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 116.0 kDa
    Observed band size: 110 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"