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LPP Recombinant Rabbit Monoclonal Antibody [JE55-29]

Usd: 385

Specification

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Catalog# ET7111-02

LPP Recombinant Rabbit Monoclonal Antibody [JE55-29]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

LPP Recombinant Rabbit Monoclonal Antibody [JE55-29]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human LPP aa 1-140 / 612.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 66 kDa

Positive Control

Daudi cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Mouse small intestine tissue lysate, Rat skin tissue lysate, Mouse smooth muscle tissue lysate, rat bladder tissue, human kidney intestine tissue, human small intestine tissue.

Conjugation

unconjugated

Clone Number

JE55-29

RRID

AB_3071059

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

Target

Function

This gene encodes a member of a subfamily of LIM domain proteins that are characterized by an N-terminal proline-rich region and three C-terminal LIM domains. The encoded protein localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility. This protein also shuttles through the nucleus and may function as a transcriptional co-activator. This gene is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth. Alternative splicing results in multiple transcript variants.

Background References

1. Asmar AT. et. al. Lpp, the Braun lipoprotein, turns 50-major achievements and remaining issues. FEMS Microbiol Lett. 2018 Sep.

2. Ngan E. et. al. Emerging roles for LPP in metastatic cancer progression. J Cell Commun Signal. 2018 Mar.

Sequence Similarity

Belongs to the zyxin/ajuba family.

Tissue Specificity

Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes.

Subcellular Location

Nucleus, cell membrane, cytoplasm, cell junction.

UNIPROT

SwissProt: Q93052 Human

SwissProt: Q8BFW7 Mouse

SwissProt: Q5XI07 Rat

Synonyms

DKFZp779O0231 antibody

FLJ30652 antibody

FLJ41512 antibody

LIM domain containing preferred translocation partner in lipoma antibody

LIM domain-containing preferred translocation partner in lipoma antibody

LIM protein antibody

Lipoma preferred partner antibody

Lipoma-preferred partner antibody

lpp antibody

LPP_HUMAN antibody

Images

  • Western blot analysis of LPP on different lysates with Rabbit anti-LPP antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/1,000 dilution.<br /><br />Lane 1: Daudi cell lysate (20 µg/Lane)<br />Lane 2: HeLa cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: Mouse small intestine tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 66 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LPP on different lysates with Rabbit anti-LPP antibody (ET7111-02) at 1/1,000 dilution.

    Lane 1: Daudi cell lysate (20 µg/Lane)
    Lane 2: HeLa cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: Mouse small intestine tissue lysate (40 µg/Lane)

    Predicted band size: 66 kDa
    Observed band size: 70 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-02) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of LPP on different lysates with Rabbit anti-LPP antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/500 dilution.<br /><br />Lane 1: Rat skin tissue lysate<br />Lane 2: Mouse smooth muscle tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 66 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of LPP on different lysates with Rabbit anti-LPP antibody (ET7111-02) at 1/500 dilution.

    Lane 1: Rat skin tissue lysate
    Lane 2: Mouse smooth muscle tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 66 kDa
    Observed band size: 75 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-02) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-LPP antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue with Rabbit anti-LPP antibody (ET7111-02) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-02) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney intestine tissue with Rabbit anti-LPP antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney intestine tissue with Rabbit anti-LPP antibody (ET7111-02) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-02) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-LPP antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-02" style="font-weight: bold;text-decoration: underline;">ET7111-02</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-LPP antibody (ET7111-02) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-02) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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