GTF2F2 Recombinant Rabbit Monoclonal Antibody [JE56-15]
Catalog# ET7111-08
GTF2F2 Recombinant Rabbit Monoclonal Antibody [JE56-15]
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
GTF2F2 Recombinant Rabbit Monoclonal Antibody [JE56-15]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within C-terminal Human GTF2F2.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 28 kDa
Positive Control
A549 cell lysate, HepG2 cell lysate, C2C12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human skin tissue, human placenta tissue, human uterus tissue, rat testis tissue, mouse testis tissue, HepG2.
Conjugation
unconjugated
Clone Number
JE56-15
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:50
Target
Function
TFIIF RAP 30 Antibody is a high quality monoclonal TFIIF RAP 30 antibody (also designated TFIIF RAP 30 antibody) suitable for the detection of the TFIIF RAP 30 protein of mouse, rat and human origin. TFIIF RAP 30 Antibody is available as both the non-conjugated anti-TFIIF RAP 30 antibody form, as well as multiple conjugated forms of anti-TFIIF RAP 30 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
Background References
1. Minchenko OH. et. al. Hypoxic regulation of MYBL1, MEST, TCF3, TCF8, GTF2B, GTF2F2 and SNAI2 genes expression in U87 glioma cells upon IRE1 inhibition. Ukr Biochem J. 2016 Nov-Dec
Subcellular Location
Nucleus.
Synonyms
ATP dependent helicase GTF2F2 antibody
ATP-dependent helicase GTF2F2 antibody
BTF4 antibody
General transcription factor IIF 30 kDa subunit antibody
General transcription factor IIF polypeptide 2 antibody
General transcription factor IIF subunit 2 antibody
General transcription factor IIF, polypeptide 2, 30kDa antibody
Gtf2f2 antibody
RAP30 antibody
T2FB_HUMAN antibody
ExpandImages
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Western blot analysis of GTF2F2 on different lysates with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/5,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: C2C12 cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (20 µg/Lane)
Lane 5: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-08) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-08) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HepG2 cells labeling GTF2F2 with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GTF2F2 antibody (ET7111-08) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"