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HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]

Usd: 385

Specification

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Catalog# ET7111-14

HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant fragment within N-terminal Human HTF9C.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Cell

Target Molecular Weight

Predicted band size: 69 kDa

Positive Control

293 cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human liver carcinoma tissue, human thyroid tissue, human breast carcinoma tissue, 293.

Conjugation

unconjugated

Clone Number

JE55-60

RRID

AB_3071071

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • IF-Cell

  • 1:100

Target

Function

The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.

Background References

1. Chang YH. et. al. TRMT2A is a novel cell cycle regulator that suppresses cell proliferation. Biochem Biophys Res Commun. 2019 Jan

2. Hicks DG. et. al. The expression of TRMT2A, a novel cell cycle regulated protein, identifies a subset of breast cancer patients with HER2 over-expression that are at an increased risk of recurrence. BMC Cancer. 2010 Mar

Subcellular Location

Nucleoplasm and Cytosol.(Predicted)

UNIPROT

SwissProt: Q8IZ69 Human

Synonyms

HpaII tiny fragments locus 9c protein antibody

HTF9C antibody

TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody

TRM2 tRNA methyltransferase 2 homolog A antibody

TRM2A_HUMAN antibody

TRMT2A antibody

tRNA (uracil-5-)-methyltransferase homolog A antibody

tRNA methyltransferase 2 homolog A antibody

tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody

Images

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-14" style="font-weight: bold;text-decoration: underline;">ET7111-14</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-14" style="font-weight: bold;text-decoration: underline;">ET7111-14</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-14" style="font-weight: bold;text-decoration: underline;">ET7111-14</a>, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of 293 cells labeling HTF9C with Rabbit anti-HTF9C antibody (<a href="/products/ET7111-14" style="font-weight: bold;text-decoration: underline;">ET7111-14</a>) at 1/100 dilution.<br /><br />Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HTF9C antibody (<a href="/products/ET7111-14" style="font-weight: bold;text-decoration: underline;">ET7111-14</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of 293 cells labeling HTF9C with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution.

    Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Western blot analysis of HTF9C on different lysates with Rabbit anti-HTF9C antibody (<a href="/products/ET7111-14" style="font-weight: bold;text-decoration: underline;">ET7111-14</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293(Human embryonic kidney cell) cell lysate<br />Lane 2: 293T(Human embryonic kidney cell) cell lysate+<br />Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate<br />Lane 4: MCF-7 (Human breast cancer cell) cell lysate<br />Lane 5: HepG2 (Human hepatocellular carcinoma cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 25 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET7111-14" style="font-weight: bold;text-decoration: underline;">ET7111-14</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 69 kDa<br />Observed band size: 75 kDa

    Western blot analysis of HTF9C on different lysates with Rabbit anti-HTF9C antibody (ET7111-14) at 1/2,000 dilution.

    Lane 1: HEK-293(Human embryonic kidney cell) cell lysate
    Lane 2: 293T(Human embryonic kidney cell) cell lysate+
    Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 4: MCF-7 (Human breast cancer cell) cell lysate
    Lane 5: HepG2 (Human hepatocellular carcinoma cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 25 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET7111-14, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 69 kDa
    Observed band size: 75 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"