HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]
Catalog# ET7111-14
HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]
-
WB
-
IHC-P
-
IF-Cell
-
Human
-
unconjugated
Overview
Product Name
HTF9C Recombinant Rabbit Monoclonal Antibody [JE55-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant fragment within N-terminal Human HTF9C.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 69 kDa
Positive Control
293 cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human liver carcinoma tissue, human thyroid tissue, human breast carcinoma tissue, 293.
Conjugation
unconjugated
Clone Number
JE55-60
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:50-1:200
-
IF-Cell
-
1:100
Target
Function
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
Background References
1. Chang YH. et. al. TRMT2A is a novel cell cycle regulator that suppresses cell proliferation. Biochem Biophys Res Commun. 2019 Jan
2. Hicks DG. et. al. The expression of TRMT2A, a novel cell cycle regulated protein, identifies a subset of breast cancer patients with HER2 over-expression that are at an increased risk of recurrence. BMC Cancer. 2010 Mar
Subcellular Location
Nucleoplasm and Cytosol.(Predicted)
UNIPROT
Synonyms
HpaII tiny fragments locus 9c protein antibody
HTF9C antibody
TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
TRM2 tRNA methyltransferase 2 homolog A antibody
TRM2A_HUMAN antibody
TRMT2A antibody
tRNA (uracil-5-)-methyltransferase homolog A antibody
tRNA methyltransferase 2 homolog A antibody
tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody
Images
-
Western blot analysis of HTF9C on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-14, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF-7 cell lysate -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HTF9C antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-14, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunocytochemistry analysis of 293 cells labeling HTF9C with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HTF9C antibody (ET7111-14) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"