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Pirh2 Recombinant Rabbit Monoclonal Antibody [JE55-39]

Usd: 385

Specification

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Catalog# ET7111-16

Pirh2 Recombinant Rabbit Monoclonal Antibody [JE55-39]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Pirh2 Recombinant Rabbit Monoclonal Antibody [JE55-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal Human Pirh2.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 30 kDa

Positive Control

HeLa cell lysate, 293T cell lysate, RAW264.7 cell lysate.

Conjugation

unconjugated

Clone Number

JE55-39

RRID

AB_3071073

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Background References

1. Choi M. et. al. E3 ligase RCHY1 negatively regulates HDAC2. Biochem Biophys Res Commun. 2020 Jan

2. Ma-Lauer Y. et. al. p53 down-regulates SARS coronavirus replication and is targeted by the SARS-unique domain and PLpro via E3 ubiquitin ligase RCHY1. Proc Natl Acad Sci U S A. 2016 Aug

Subcellular Location

Nucleus, Nucleus speckle, Cytoplasm.

UNIPROT

SwissProt: Q96PM5 Human

SwissProt: Q9CR50 Mouse

Synonyms

Androgen receptor N terminal interacting protein antibody

Androgen receptor N-terminal-interacting protein antibody

ARNIP antibody

CH-rich-interacting match with PLAG1 antibody

CHIMP antibody

E3 ubiquitin-protein ligase Pirh2 antibody

hARNIP antibody

hPirh2 antibody

p53 induced protein with a RING H2 domain antibody

p53-induced RING-H2 protein antibody

Expand

Images

  • Western blot analysis of Pirh2 on different lysates with Rabbit anti-Pirh2 antibody (<a href="/products/ET7111-16" style="font-weight: bold;text-decoration: underline;">ET7111-16</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: RAW264.7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 30 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-16" style="font-weight: bold;text-decoration: underline;">ET7111-16</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Pirh2 on different lysates with Rabbit anti-Pirh2 antibody (ET7111-16) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: RAW264.7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 30 kDa
    Observed band size: 27 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-16) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation