SerpinB3 Recombinant Rabbit Monoclonal Antibody [JE55-93]
Catalog# ET7111-21
SerpinB3 Recombinant Rabbit Monoclonal Antibody [JE55-93]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
SerpinB3 Recombinant Rabbit Monoclonal Antibody [JE55-93]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within N-terminal Human SerpinB3.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
A431 cell lysates, human skin tissue, human esophagus tissue.
Conjugation
unconjugated
Clone Number
JE55-93
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:200
Target
Function
SCCA1 Antibody is a high quality monoclonal SCCA1 antibody (also designated SCCA1 antibody) suitable for the detection of the SCCA1 protein of human origin. SCCA1 Antibody is available as both the non-conjugated anti-SCCA1 antibody form, as well as multiple conjugated forms of anti-SCCA1 antibody, including agarose, PE, FITC and multiple Alexa Fluor® conjugates. Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine prote-ases, such as neutrophil elastase. In 1985, Dr. Jim Travis and Dr. R.W. Carrell designated an emerging family of serine protease inhibitors as the serpin fam-ily, which share homology in both primary amino acid sequence and tertiary structure. Serpins contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive. Aberrant ex-pression of serpin family members can contribute to a number of conditions, including emphysema (a-1 antitrypsin deficiency), fatal bleeding (elastase to thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers). Human chromosome position 18q21.3 contains a cluster of serpins, including a tandem duplication of the SCCA gene, plasminogen activator inhibitor type 2, and maspin. SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2), and is mainly produced as SCCA1. The human SCCA1 gene encodes a 390 amino acid protein that was originally isolated from a metastatic cervical squamous cell carcinoma.
Background References
1. Novo E. et. al. SerpinB3 Promotes Pro-fibrogenic Responses in Activated Hepatic Stellate Cells. Sci Rep. 2017 Jun
2. Sun Y. et. al. SERPINB3 and B4: From biochemistry to biology. Semin Cell Dev Biol. 2017 Feb
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
HsT1196 antibody
Protein T4 A antibody
Protein T4-A antibody
SCC antibody
SCCA antibody
SCCA PD antibody
SCCA-1 antibody
SCCA-PD antibody
SCCA1 antibody
SCCAPD antibody
ExpandImages
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Western blot analysis of SerpinB3 on A431 cell lysates with Rabbit anti-SerpinB3 antibody (ET7111-21) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-SerpinB3 antibody (ET7111-21) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-SerpinB3 antibody (ET7111-21) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-21) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"