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Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody [JE55-35]

Usd: 385

Specification

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Catalog# ET7111-24

Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody [JE55-35]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody [JE55-35]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within C-terminal Human Cytokeratin 12.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 54 kDa

Positive Control

Mouse eyeball tissue lysate, rat eyeball tissue lysate, mouse eyeball tissue, rat eyeball tissue.

Conjugation

unconjugated

Clone Number

JE55-35

RRID

AB_3071081

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P
mouse
Tested Tested
Tested Tested
rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).

Background References

1. Du X. et. al. Mutation analysis of TGFBI and KRT12 in a case of concomitant keratoconus and granular corneal dystrophy. Graefes Arch Clin Exp Ophthalmol. 2017 Sep

2. Courtney DG. et. al. CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting. Gene Ther. 2016 Jan

Subcellular Location

Intermediate filament, cytosol, extracellular exosome.

UNIPROT

SwissProt: Q99456 Human

SwissProt: Q64291 Mouse

SwissProt: Q6IFW5 Rat

Synonyms

CK12 antibody

CK-12 antibody

Cytokeratin-12 antibody

K12 antibody

K1C12_HUMAN antibody

Keratin 12 antibody

Keratin antibody

Keratin, type I cytoskeletal 12 antibody

Keratin-12 antibody

KRT12 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Cytokeratin 12 on different lysates with Rabbit anti-Cytokeratin 12 antibody (<a href="/products/ET7111-24" style="font-weight: bold;text-decoration: underline;">ET7111-24</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse eyeball tissue lysate (40 µg/Lane)<br />Lane 2: C6 cell lysate (negative) (20 µg/Lane)<br />Lane 3: Rat eyeball tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-24" style="font-weight: bold;text-decoration: underline;">ET7111-24</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Cytokeratin 12 on different lysates with Rabbit anti-Cytokeratin 12 antibody (ET7111-24) at 1/1,000 dilution.

    Lane 1: Mouse eyeball tissue lysate (40 µg/Lane)
    Lane 2: C6 cell lysate (negative) (20 µg/Lane)
    Lane 3: Rat eyeball tissue lysate (40 µg/Lane)

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 3 minutes; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-Cytokeratin 12 antibody (<a href="/products/ET7111-24" style="font-weight: bold;text-decoration: underline;">ET7111-24</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-24" style="font-weight: bold;text-decoration: underline;">ET7111-24</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-Cytokeratin 12 antibody (ET7111-24) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-Cytokeratin 12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-24" style="font-weight: bold;text-decoration: underline;">ET7111-24</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat eyeball tissue using anti-Cytokeratin 12 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"