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Desmocollin 3 Recombinant Rabbit Monoclonal Antibody [JE55-86]

Usd: 385

Specification

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Catalog# ET7111-26

Desmocollin 3 Recombinant Rabbit Monoclonal Antibody [JE55-86]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Desmocollin 3 Recombinant Rabbit Monoclonal Antibody [JE55-86]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal Human Desmocollin 3.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 100 kDa

Positive Control

A431 cell lysate, HCT 116 cell lysate, mouse skin tissue lysate, rat skin tissue lysate.

Conjugation

unconjugated

Clone Number

JE55-86

RRID

AB_3071083

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are a cause of hypotrichosis and recurrent skin vesicles disorder. The protein can act as an autoantigen in pemphigus diseases, and it is also considered to be a biomarker for some cancers. Alternative splicing of this gene results in multiple transcript variants.

Background References

1. Meltzanidou P. et. al. Detection of IgG Autoantibodies against Desmocollin-3 in Greek Patients with Pemphigus. Acta Dermatovenerol Croat. 2019 Mar

2. Hart ML. et. al. Expression of Desmoglein 2, Desmocollin 3 and Plakophilin 2 in Placenta and Bone Marrow-Derived Mesenchymal Stromal Cells. Stem Cell Rev Rep. 2017 Apr

Subcellular Location

Cell membrane, desmosome.

UNIPROT

SwissProt: Q14574 Human

SwissProt: P55850 Mouse

Entrez Gene: 307563 Rat

Synonyms

Cadherin family member 3 antibody

CDHF3 antibody

desmocollin 3a/3b antibody

Desmocollin 4 antibody

Desmocollin-3 antibody

Desmocollin-4 antibody

Desmocollin3 antibody

Desmocollin4 antibody

DSC antibody

DSC1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Desmocollin 3 on different lysates with Rabbit anti-Desmocollin 3 antibody (<a href="/products/ET7111-26" style="font-weight: bold;text-decoration: underline;">ET7111-26</a>) at 1/2,000 dilution.<br /><br />Lane 1: A431 cell lysate (RIPA lysis)<br />Lane 2: HCT 116 cell lysate (low expression) (RIPA lysis)<br />Lane 3: Mouse skin tissue lysate (RIPA lysis)<br />Lane 4: Mouse skin tissue lysate (hot lysis)<br />Lane 5: Rat skin tissue lysate (hot lysis)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 100 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-26" style="font-weight: bold;text-decoration: underline;">ET7111-26</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Desmocollin 3 on different lysates with Rabbit anti-Desmocollin 3 antibody (ET7111-26) at 1/2,000 dilution.

    Lane 1: A431 cell lysate (RIPA lysis)
    Lane 2: HCT 116 cell lysate (low expression) (RIPA lysis)
    Lane 3: Mouse skin tissue lysate (RIPA lysis)
    Lane 4: Mouse skin tissue lysate (hot lysis)
    Lane 5: Rat skin tissue lysate (hot lysis)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 100 kDa
    Observed band size: 100 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-26) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation