EpCAM Mouse Monoclonal Antibody [7E11]
Catalog# EM1712-43
EpCAM Mouse Monoclonal Antibody [7E11]
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WB
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IHC-P
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ELISA
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Human
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unconjugated
Overview
Product Name
EpCAM Mouse Monoclonal Antibody [7E11]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Purified recombinant fragment of human EPCAM expressed in E. Coli.
Species Reactivity
Human
Validated Applications
WB, IHC-P, ELISA
Molecular Weight
35 kDa
Positive Control
HTC116, T47D, human lung cance, colon cancer, breast cancer, rectal cancer.
Conjugation
unconjugated
Clone Number
7E11
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:200-1:1,000
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ELISA
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1:10,000
Target
Function
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.Tissue specificity: This protein is expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas.ABCAM:Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.BIOLEGEND:This cell surface, glycosyl;ated 40kD protein is highly expressed in the bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin.
Background References
1. Muenz M. et. al. The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation. Oncogene 23:5748-5758(2004).
Subcellular Location
Cell junction, Cell membrane, Membrane, Tight junction.
UNIPROT
Synonyms
17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
Cell surface glycoprotein Trop 2 antibody
ExpandImages
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Western blot analysis using EPCAM mouse mAb against HTC116 (1) and T47D (2) cell lysate.
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Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), breast cancer (C) and rectal cancer(D), using EPCAM mouse mAb with DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Products with the same target and pathway
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